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Literature summary extracted from

  • Kim, H.J.; Lim, B.C.; Yeom, S.J.; Kim, Y.S.; Kim, D.; Oh, D.K.
    Roles of Ile66 and Ala107 of D-psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its substrate, D-fructose (2009), Biotechnol. Lett., 32, 113-118.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.B12 A107I 7.7fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose Agrobacterium tumefaciens
5.1.3.B12 A107P 1.4fold decrease in kcat/Km for D-fructose, 6.6fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 A107V 13.9fold decrease in kcat/Km for D-fructose, no activity detected with D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66A 1.4fold increase in kcat/Km for D-fructose, 150fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66C 3fold decrease in kcat/Km for D-fructose, 29.2fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66F 2.1fold decrease in kcat/Km for D-fructose, 23.3fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66G 1.9fold decrease in kcat/Km for D-fructose, 87.5fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66L 1.6fold increase in kcat/Km for D-fructose, 58,3fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66V 1.9fold decrease in kcat/Km for D-fructose, 24fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66W 1.4fold decrease in kcat/Km for D-fructose, 42fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens
5.1.3.B12 I66Y 3fold decrease in kcat/Km for D-fructose, 26fold decrease in kcat/Km for D-tagatose Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.B12 1 7 D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 3 5 D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 6 11 D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 11
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
5.1.3.B12 16
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 19
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 22
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
5.1.3.B12 22
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 28
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 33
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 36
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
5.1.3.B12 67
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
5.1.3.B12 91
-
 D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 140
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
5.1.3.B12 213
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 429
-
 D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
5.1.3.B12 694
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 696
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 1146
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 1369
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 1408
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
5.1.3.B12 1640
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.B12 Agrobacterium tumefaciens A9CH28
-
-
5.1.3.B12 Agrobacterium tumefaciens ATCC 33970 A9CH28
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.B12
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.B12 D-fructose
-
 Agrobacterium tumefaciens D-psicose
-
 r
5.1.3.B12 D-fructose
-
 Agrobacterium tumefaciens ATCC 33970 D-psicose
-
 r
5.1.3.B12 D-psicose
-
 Agrobacterium tumefaciens D-fructose
-
 r
5.1.3.B12 D-psicose
-
 Agrobacterium tumefaciens ATCC 33970 D-fructose
-
 r
5.1.3.B12 D-sorbose
-
 Agrobacterium tumefaciens D-tagatose
-
 r
5.1.3.B12 D-sorbose
-
 Agrobacterium tumefaciens ATCC 33970 D-tagatose
-
 r
5.1.3.B12 D-tagatose
-
 Agrobacterium tumefaciens D-sorbose
-
 r
5.1.3.B12 D-tagatose
-
 Agrobacterium tumefaciens ATCC 33970 D-sorbose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.B12 Atu4750 locus name Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.B12 45
-
 assay at Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.B12 11
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 23
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
5.1.3.B12 26
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
5.1.3.B12 33
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 44
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 50
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
5.1.3.B12 56
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 62
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 64
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 72
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 88
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
5.1.3.B12 88
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 100
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 116
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 136
-
 D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
5.1.3.B12 152
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 168
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 176
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
5.1.3.B12 194
-
 D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 308
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
5.1.3.B12 341
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 996
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.B12 8
-
 assay at Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.B12 0.014
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
5.1.3.B12 0.024
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 0.036
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens
5.1.3.B12 0.05
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 0.072
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 0.08
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 0.087
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 0.09
-
 D-tagatose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 0.32
-
 D-tagatose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
5.1.3.B12 0.72
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107V Agrobacterium tumefaciens
5.1.3.B12 1.3
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107I Agrobacterium tumefaciens
5.1.3.B12 2.1
-
 D-tagatose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 3.3
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66C Agrobacterium tumefaciens
5.1.3.B12 3.3
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66Y Agrobacterium tumefaciens
5.1.3.B12 4.8
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66F Agrobacterium tumefaciens
5.1.3.B12 5.2
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66V Agrobacterium tumefaciens
5.1.3.B12 5.4
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66G Agrobacterium tumefaciens
5.1.3.B12 7
-
 D-fructose pH 8.0, 45°C, mutant enzyme A107P Agrobacterium tumefaciens
5.1.3.B12 7.3
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66W Agrobacterium tumefaciens
5.1.3.B12 10
-
 D-fructose pH 8.0, 45°C, wild-type enzyme Agrobacterium tumefaciens
5.1.3.B12 14
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66A Agrobacterium tumefaciens
5.1.3.B12 16
-
 D-fructose pH 8.0, 45°C, mutant enzyme I66L Agrobacterium tumefaciens