BRENDA - Enzyme Database

Cloning and expression of the isocitrate lyase gene from a nitrogen-fixing bacterium, Azotobacter vinelandii, and functional analysis of the enzyme by site-directed mutagenesis

Tanaka, Y.; Hayashi, T.; Yamaoka, N.; Takada, Y.; Biosci. Biotechnol. Biochem. 76, 78-83 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
gene icl, expression of N-terminally His6-tagged enzyme in Escherichia coli strains XL-1 Blue, KM22 (a DELTArecBCD mutant), and ACA421, the latter is defective in isocitrate lyase
Azotobacter vinelandii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Azotobacter vinelandii
-
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Azotobacter vinelandii IAM1078
-
succinate + glyoxylate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Azotobacter vinelandii
A8C781
gene icl
-
4.1.3.1
Azotobacter vinelandii IAM1078
A8C781
gene icl
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
Azotobacter vinelandii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
culture condition:acetate-grown cell
cell growth is similar in glucose- or sodium acetate-medium, but the enzyme activity is 43fold higher in acetate-grown cells
Azotobacter vinelandii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
727196
Azotobacter vinelandii
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
727196
Azotobacter vinelandii IAM1078
succinate + glyoxylate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
assay at
Azotobacter vinelandii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
the amino acid residue at position 504 is involved in thermostability of the ICL enzymes
Azotobacter vinelandii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.8
-
assay at
Azotobacter vinelandii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
gene icl, expression of N-terminally His6-tagged enzyme in Escherichia coli strains XL-1 Blue, KM22 (a DELTArecBCD mutant), and ACA421, the latter is defective in isocitrate lyase
Azotobacter vinelandii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Azotobacter vinelandii
-
succinate + glyoxylate
-
-
?
4.1.3.1
isocitrate
Azotobacter vinelandii IAM1078
-
succinate + glyoxylate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
Azotobacter vinelandii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
culture condition:acetate-grown cell
cell growth is similar in glucose- or sodium acetate-medium, but the enzyme activity is 43fold higher in acetate-grown cells
Azotobacter vinelandii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
727196
Azotobacter vinelandii
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
727196
Azotobacter vinelandii IAM1078
succinate + glyoxylate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
assay at
Azotobacter vinelandii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
additional information
-
the amino acid residue at position 504 is involved in thermostability of the ICL enzymes
Azotobacter vinelandii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.8
-
assay at
Azotobacter vinelandii
Expression
EC Number
Organism
Commentary
Expression
4.1.3.1
Azotobacter vinelandii
enzyme expression is induced by acetate
up
General Information
EC Number
General Information
Commentary
Organism
4.1.3.1
evolution
the enzyme belongs to the isocitrate lyase ICL subfamily 3, one of two eubacterial groups, it has a substitution of M504I compared to the cold-adapted ICLs, rendering is less thermostable, phylogenetic analysis, overview
Azotobacter vinelandii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.3.1
evolution
the enzyme belongs to the isocitrate lyase ICL subfamily 3, one of two eubacterial groups, it has a substitution of M504I compared to the cold-adapted ICLs, rendering is less thermostable, phylogenetic analysis, overview
Azotobacter vinelandii
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.1.3.1
Azotobacter vinelandii
enzyme expression is induced by acetate
up