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Literature summary extracted from

  • Ojima, T.; Saburi, W.; Sato, H.; Yamamoto, T.; Mori, H.; Matsui, H.
    Biochemical characterization of a thermophilic cellobiose 2-epimerase from a thermohalophilic bacterium, Rhodothermus marinus JCM9785 (2011), Biosci. Biotechnol. Biochem., 75, 2162-2168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 gene RmCE, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Rhodothermus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.11 27.2
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
5.1.3.11 28.8
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.11 cellobiose Rhodothermus marinus
-
 D-glucosyl-D-mannose
-
 r
5.1.3.11 cellobiose Rhodothermus marinus JCM 9785
-
 D-glucosyl-D-mannose
-
 r
5.1.3.11 additional information Rhodothermus marinus cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides ?
-
 ?
5.1.3.11 additional information Rhodothermus marinus JCM 9785 cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Rhodothermus marinus F8WRK9 gene RmCE
-
5.1.3.11 Rhodothermus marinus JCM 9785 F8WRK9 gene RmCE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11 recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, ultrafiltration, and gel filtration Rhodothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 4-O-beta-D-glucosyl-D-mannose
-
 Rhodothermus marinus 4-O-beta-D-galactosyl-D-mannose
-
 r
5.1.3.11 4-O-beta-D-glucosyl-D-mannose
-
 Rhodothermus marinus JCM 9785 4-O-beta-D-galactosyl-D-mannose
-
 r
5.1.3.11 cellobiose
-
 Rhodothermus marinus D-glucosyl-D-mannose
-
 r
5.1.3.11 cellobiose
-
 Rhodothermus marinus JCM 9785 D-glucosyl-D-mannose
-
 r
5.1.3.11 lactose
-
 Rhodothermus marinus epilactose
-
 r
5.1.3.11 lactose
-
 Rhodothermus marinus JCM 9785 epilactose
-
 r
5.1.3.11 additional information cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides Rhodothermus marinus ?
-
 ?
5.1.3.11 additional information substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose Rhodothermus marinus ?
-
 ?
5.1.3.11 additional information cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides Rhodothermus marinus JCM 9785 ?
-
 ?
5.1.3.11 additional information substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose Rhodothermus marinus JCM 9785 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.11 cellobiose 2-epimerase
-
 Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 80
-
-
 Rhodothermus marinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.11 50 90 high activity within this range Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.11 80
-
 purified recombinant enzyme, stable up to Rhodothermus marinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.11 80.8
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
5.1.3.11 111
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 6.3
-
-
 Rhodothermus marinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.11 3.2 10.8 purified recombinant enzyme, stable at Rhodothermus marinus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.11 Rhodothermus marinus sequence calculation
-
 6.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.11 2.97
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
5.1.3.11 3.85
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus