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Literature summary extracted from

  • Li, Z.; Li, C.; Li, J.; Zhang, Y.
    Molecular cloning and functional characterization of two divergent 4-coumarate: coenzyme a ligases from Kudzu (Pueraria lobata) (2014), Biol. Pharm. Bull., 37, 113-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pET28a, subcloning in Escherichia coli strain M15 Pueraria montana var. lobata
6.2.1.12 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)/pQE30, subcloning in Escherichia coli strain M15 Pueraria montana var. lobata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.0035
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.0091
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.0149
-
 caffeate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.0884
-
 ferulate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.141
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.664
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Pueraria montana var. lobata
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA Pueraria montana var. lobata
-
 AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA Pueraria montana var. lobata
-
 AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + trans-cinnamate + CoA Pueraria montana var. lobata
-
 AMP + diphosphate + trans-cinnamoyl-CoA
-
 ?
6.2.1.12 additional information Pueraria montana var. lobata isozyme Pl4CL2 has a broader substrate specificity than Pl4CL1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Pueraria montana var. lobata U3MJP5 isozyme Pl4CL2
-
6.2.1.12 Pueraria montana var. lobata U3MNX0 isozyme Pl4CL1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 additional information no N-terminal extension of Pl4CL2 Pueraria montana var. lobata
-
6.2.1.12 additional information Pl4CL1 has an N-terminal extension of twenty-one amino acid residues Pueraria montana var. lobata
-
6.2.1.12 root
-
 Pueraria montana var. lobata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Pueraria montana var. lobata AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Pueraria montana var. lobata AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Pueraria montana var. lobata AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + trans-cinnamate + CoA
-
 Pueraria montana var. lobata AMP + diphosphate + trans-cinnamoyl-CoA
-
 ?
6.2.1.12 additional information isozyme Pl4CL2 has a broader substrate specificity than Pl4CL1 Pueraria montana var. lobata ?
-
 ?
6.2.1.12 additional information no activity with sinapate Pueraria montana var. lobata ?
-
 ?
6.2.1.12 additional information no activity with sinapate, ferulate and caffeate Pueraria montana var. lobata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate: coenzyme A ligase
-
 Pueraria montana var. lobata
6.2.1.12 Pl4CL1
-
 Pueraria montana var. lobata
6.2.1.12 Pl4CL2
-
 Pueraria montana var. lobata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 22
-
 assay at room temperature Pueraria montana var. lobata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 0.47
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.57
-
 ferulate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.92
-
 caffeate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 0.97
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 1.02
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 1.28
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.8
-
 assay at Pueraria montana var. lobata

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Pueraria montana var. lobata

Expression

EC Number Organism Comment Expression
6.2.1.12 Pueraria montana var. lobata expression of Pl4CL2 is not significantly influenced in methyl jasmonate-treated samples additional information
6.2.1.12 Pueraria montana var. lobata expression of Pl4CL1 is increased more than 3fold in the 24 h methyl jasmonate-treated samples up

General Information

EC Number General Information Comment Organism
6.2.1.12 evolution isozyme Pl4CL1 belongs to the class II 4-coumarate-CoA ligases Pueraria montana var. lobata
6.2.1.12 evolution isozyme Pl4CL2 belongs to the class I 4-coumarate-CoA ligases Pueraria montana var. lobata
6.2.1.12 physiological function isozyme Pl4CL1 might play a role in isoflavone biosynthesis Pueraria montana var. lobata
6.2.1.12 physiological function isozyme Pl4CL2 might function as a housekeeping enzyme concerning lignification Pueraria montana var. lobata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.12 1.46
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 3.3
-
 trans-cinnamate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 6.5
-
 ferulate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 62
-
 caffeate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 112
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata
6.2.1.12 364
-
 4-coumarate pH 7.8, 22°C Pueraria montana var. lobata