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Literature summary extracted from
- Reaction coupling through interdomain contacts in imidazole glycerol phosphate synthase (2005), Biochemistry, 44, 11974-11985.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.3.1.B2 | D359A | 2300fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 4fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 7.5fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
| 4.3.1.B2 | K196A | 0.43fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 1fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 3fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
| 4.3.1.B2 | K196A/D359A | 2300fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 4fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
| 4.3.1.B2 | N13A | 130fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 3fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 350fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
| 4.3.1.B2 | Q397A | 7.5fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 1fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 18fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.B2 | 0.00027 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A | Saccharomyces cerevisiae |  |
| 4.3.1.B2 | 0.0013 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.0036 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.005 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.0055 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.0141 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.026 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.03 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.034 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.055 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.071 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.109 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.49 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1 | 4 | L-glutamine | pH 8.0, 30°C, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1.8 | - |
L-glutamine | pH 8.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 2.3 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 4.8 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 7 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme Q397A | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.B2 | Saccharomyces cerevisiae | P33734 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.B2 | L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Saccharomyces cerevisiae | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
| 4.3.1.B2 | additional information | the enzyme also shows glutaminase activity. The glutaminase activity of the glutaminase domain is tightly regulated by the acceptor substrate domain. In IGP synthase the glutaminase and N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate binding sites are separated by 30 A. Using kinetic analyses of site-specific mutants and molecular dynamic simulations, it is determined that an interdomain salt bridge in IGP synthase between D359 and K196 (approximately 16 A from the N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate binding site) plays a key role in mediating communication between the two active sites. This interdomain contact modulates the glutaminase loop containing the histidine and glutamic acid of the catalytic triad to control glutamine hydrolysis | Saccharomyces cerevisiae | ? | - |
? | |
| 4.3.1.B2 | NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Saccharomyces cerevisiae | D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.1.B2 | IGP synthase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.B2 | 30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.B2 | 0.00029 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.0059 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.007 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.007 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.01 | 1 | N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.019 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.11 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.35 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.38 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.39 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.46 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.56 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.6 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.74 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.845 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1.4 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 5.4 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 6.9 | - |
L-glutamine | pH 8.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.B2 | 7 | - |
assay at | Saccharomyces cerevisiae |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.B2 | 0.00042 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.0032 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.004 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.016 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.21 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.51 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 0.52 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1.2 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1.2 | - |
L-glutamine | pH 8.0, 30°C, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 3.6 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 3.8 | - |
L-glutamine | pH 8.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 3.8 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 4.9 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 9 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 14 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 15 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 1200 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae | |
| 4.3.1.B2 | 2800 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A | Saccharomyces cerevisiae | |
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