EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.1.B2 | K258A | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 43:1 (wild-type ratio is 1:1), 2600fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 385fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1055fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | K258R | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 3:1 (wild-type ratio is 1:1), 20fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 125fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 45fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | K360R | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 1:1 (identical to wild-type ratio), 1090fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 3.5fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 6.4fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | R239A | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 122:1 (wild-type ratio is 1:1), 860fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 5.4fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 3450fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | R239H | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 154:1 (wild-type ratio is 1:1), 2400fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 5.2fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1360fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | R239K | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 40:1 (wild-type ratio is 1:1), 218fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 3.9fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 540fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
4.3.1.B2 | R360A | ratio of glutamine turnover to N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate turnover is 3:1 (wild-type ratio is 1:1), 9.2fold decrease in kcat/KM of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate L-glutamine), 1.4fold decrease in kcat/Km N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 15fold decrease in kcat/Km of L-glutamine | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.B2 | albizziin | mixed type inhibition versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, competitive inhibition versus L-glutamine | Saccharomyces cerevisiae | |
4.3.1.B2 | N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | competitive versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mixed type inhibition versus L-glutamine | Saccharomyces cerevisiae | |
4.3.1.B2 | N1-[(5'-phosphoribitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | competitive versus N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.0016 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.0018 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.002 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.0023 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.003 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.005 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.008 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.046 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.053 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.055 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.065 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.072 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.075 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.08 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.098 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.139 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.3 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.8 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.9 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.9 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.96 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 2.1 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 6.5 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 6.5 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.B2 | Saccharomyces cerevisiae | P33734 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.B2 | - |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.B2 | L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Saccharomyces cerevisiae | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
4.3.1.B2 | additional information | the glutaminase activity of the enzyme is stimulated 4900fold in the presence of the acceptor substrate N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae | ? | - |
? | |
4.3.1.B2 | NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Saccharomyces cerevisiae | D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.B2 | IGP synthase | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.B2 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.025 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.045 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.126 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.15 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.16 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.21 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.24 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.29 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.3 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.31 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.49 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.7 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.845 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.13 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 3.9 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 4.3 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 5 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 5.4 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 5.4 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 6 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 6.9 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 6.9 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 8.7 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 9 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.B2 | 7 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.00067 | - |
N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | pH 7.0, 30°C, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.0016 | - |
N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | pH 7.0, 30°C, substrate: L-glutamine, uncompetitive inhibition constant | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.003 | - |
N1-[(5'-phosphoarabinitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | pH 7.0, 30°C, substrate: L-glutamine, competitive inhibition constant | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.004 | - |
N1-[(5'-phosphoribitolyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | pH 7.0, 30°C, N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.38 | - |
albizziin | pH 7.0, 30°C, substrate: L-glutamine | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.6 | - |
albizziin | pH 7.0, 30°C, competitive inhibition constant, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.6 | - |
albizziin | pH 7.0, 30°C, uncompetitive inhibition constant, substrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.B2 | 0.0011 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.0028 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.0036 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.007 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.039 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.084 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.12 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.247 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.46 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258A | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.5 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 0.59 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 1.4 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 2.8 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239A | Saccharomyces cerevisiae | |
4.3.1.B2 | 2.9 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239H | Saccharomyces cerevisiae | |
4.3.1.B2 | 3.8 | - |
L-glutamine | pH 7.0, 30°C, cosubstrate: N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 3.8 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 4.3 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 5.5 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme R239K | Saccharomyces cerevisiae | |
4.3.1.B2 | 11 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 15 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: NH4+, wild-type enzyme | Saccharomyces cerevisiae | |
4.3.1.B2 | 60 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K258R | Saccharomyces cerevisiae | |
4.3.1.B2 | 110 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360R | Saccharomyces cerevisiae | |
4.3.1.B2 | 130 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K360A | Saccharomyces cerevisiae | |
4.3.1.B2 | 1200 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 7.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme | Saccharomyces cerevisiae |