Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tan, M.; Zhu, B.; Liu, R.J.; Chen, X.; Zhou, X.L.; Wang, E.D.
    Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase (2013), Biochem. J., 449, 123-131.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 E184A the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme Escherichia coli
6.1.1.4 E184R the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme. The substitution specifically inhibits tRNA-dependent pre-transfer editing Escherichia coli
6.1.1.4 E184R/T252R the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme Escherichia coli
6.1.1.4 R185E the mutation significantly enhances tRNA-dependent pre-transfer editing activity Escherichia coli
6.1.1.4 R286E the mutation significantly enhances tRNA-dependent pre-transfer editing activity Escherichia coli
6.1.1.4 T252R the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.002
-
 Ile-tRNALeu mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 0.0021
-
 Ile-tRNALeu wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 0.0024
-
 Ile-tRNALeu mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 0.0025
-
 Ile-tRNALeu mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 0.039
-
 L-leucine wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Escherichia coli
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information Escherichia coli the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Escherichia coli P07813
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 AMP + diphosphate + Ile-tRNALeu
-
 Escherichia coli ATP + L-isoleucine + tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
 Escherichia coli
6.1.1.4 LeuRS
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 7
-
 Ile-tRNALeu wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 7.4
-
 Ile-tRNALeu mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 7.6
-
 Ile-tRNALeu mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 8.8
-
 Ile-tRNALeu mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 73.7
-
 L-leucine wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.4 1890
-
 L-leucine wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 3100
-
 Ile-tRNALeu mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 3300
-
 Ile-tRNALeu wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 3500
-
 Ile-tRNALeu mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli
6.1.1.4 3800
-
 Ile-tRNALeu mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C Escherichia coli