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Literature summary extracted from

  • Sun, H.; Li, Y.; Feng, S.; Zou, W.; Guo, K.; Fan, C.; Si, S.; Peng, L.
    Analysis of five rice 4-coumarate:coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice (2013), Biochem. Biophys. Res. Commun., 430, 1151-1156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 isozyme Os4Cl1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa
6.2.1.12 isozyme Os4Cl2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa
6.2.1.12 isozyme Os4Cl3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa
6.2.1.12 isozyme Os4Cl4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa
6.2.1.12 isozyme Os4Cl5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.079
-
 ferulate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.103
-
 4-coumarate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.136
-
 4-coumarate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.143
-
 4-coumarate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.154
-
 caffeate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.18
-
 ferulate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.212
-
 4-coumarate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.234
-
 caffeate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.262
-
 caffeate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.282
-
 4-coumarate pH not specified in the publication, 30°C, isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.282
-
 caffeate pH not specified in the publication, 30°C, isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.309
-
 ferulate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa
6.2.1.12 0.38
-
 ferulate pH not specified in the publication, 30°C, isozyme Os4Cl3 Oryza sativa
6.2.1.12 0.384
-
 ferulate pH not specified in the publication, 30°C, isozyme Os4Cl1 Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Oryza sativa
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA Oryza sativa
-
 AMP + diphosphate + feruloyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Oryza sativa P17814 Os4Cl1; ssp. japonica, isozyme Os4Cl1
-
6.2.1.12 Oryza sativa Q42982 Os4Cl2; ssp. japonica, isozyme Os4Cl2
-
6.2.1.12 Oryza sativa Q67W82 Os4Cl4; ssp. japonica, isozyme Os4Cl4
-
6.2.1.12 Oryza sativa Q6ETN3 Os4Cl3; ssp. japonica, isozyme Os4Cl3
-
6.2.1.12 Oryza sativa Q6ZAC1 Os4Cl5; ssp. japonica, isozyme Os4Cl5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant GST-tagged enzyme from Escherichia coli strain BL21 by affinity chromatography and removal of the GST-tag Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 anther isozyme Os4CL2 is specifically expressed in the anther Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Oryza sativa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA best substrate Oryza sativa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA low activity Oryza sativa AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Oryza sativa AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information no activity with sinapate Oryza sativa ?
-
 ?
6.2.1.12 additional information no activity with sinapate and caffeate Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-Coumarate:coenzyme A ligase
-
 Oryza sativa
6.2.1.12 4CL
-
 Oryza sativa
6.2.1.12 Os4Cl1
-
 Oryza sativa
6.2.1.12 Os4Cl2
-
 Oryza sativa
6.2.1.12 Os4Cl3
-
 Oryza sativa
6.2.1.12 Os4Cl4
-
 Oryza sativa
6.2.1.12 Os4Cl5
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 30
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Oryza sativa

Expression

EC Number Organism Comment Expression
6.2.1.12 Oryza sativa isozyme Os4CL1 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light down
6.2.1.12 Oryza sativa isozyme Os4CL3 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light down
6.2.1.12 Oryza sativa isozyme Os4CL4 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light down
6.2.1.12 Oryza sativa isozyme Os4CL5 is downregulated by UV radiation when dark-adapted rice plants are illuminated with UV-containing white light down
6.2.1.12 Oryza sativa Os4CL2 is strongly activated by UV irradiation, the isozyme is induced by up to 16fold at 12 h after the onset of irradiation, and expression remains high at 24 h after treatment up

General Information

EC Number General Information Comment Organism
6.2.1.12 additional information the existence of a valine residue at the substrate-binding pocket may mainly affect rice enzyme activity toward sinapic acid. The amino acid sequence similarities within the Os4CL gene family share a percentage of identity between 56% and 84%, positions of the four introns in these genes are conserved, but the introns differ in length and sequence, sequence comparisons of rice isozymes, overview Oryza sativa
6.2.1.12 additional information the existence of a valine residue at the substrate-binding pocket may mainly affect the rice enzyme activity toward sinapic acid. The amino acid sequence similarities within the Os4CL gene family share a percentage of identity between 56% and 84%, positions of the four introns in these genes are conserved, but the introns differ in length and sequence, sequence comparisons of rice isozymes, overview Oryza sativa
6.2.1.12 physiological function the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of flavonoids Oryza sativa
6.2.1.12 physiological function the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of flavonoids. Isozyme Os4Cl shows potential involvement in flavonoid formation Oryza sativa
6.2.1.12 physiological function the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of lignin Oryza sativa
6.2.1.12 physiological function the isozyme catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of lignin, 4-coumaric acid and ferulic acid are the two main substrates of the enzyme for monolignol biosynthesis in rice Oryza sativa