EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.11 | expressed in Escherichia coli strain KL229 | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.11 | 0.0008628 | - |
tRNASer | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 0.18 | - |
L-serine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 2 | 3.4 | L-threonine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 2.35 | - |
DL-Serine hydroxamate | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 29 | - |
L-cysteine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.11 | cytosol | the enzyme is localized exclusively in the cytosol | Zea mays | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.11 | ATP + L-serine + tRNASer | Zea mays | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? | |
6.1.1.11 | additional information | Zea mays | the enzyme shows pre-transfer editing activity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.11 | Zea mays | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.11 | ATP + DL-serine hydroxamate + tRNASer | very weak activity | Zea mays | ? | - |
? | |
6.1.1.11 | ATP + L-cysteine + tRNASer | very weak activity | Zea mays | AMP + diphosphate + L-cysteinyl-tRNASer | - |
? | |
6.1.1.11 | ATP + L-serine + tRNASer | - |
Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
6.1.1.11 | ATP + L-serine + tRNASer | tRNASer from Escherichia coli | Zea mays | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
6.1.1.11 | ATP + L-threonine + tRNASer | very weak activity | Zea mays | AMP + diphosphate + L-threonyl-tRNASer | - |
? | |
6.1.1.11 | additional information | the enzyme shows pre-transfer editing activity | Zea mays | ? | - |
? | |
6.1.1.11 | additional information | Gly73 is a major identity element recognized by the enzyme | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.11 | SerRS | - |
Zea mays |
6.1.1.11 | Seryl-tRNA synthetase | - |
Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.11 | 0.078 | - |
L-cysteine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 0.14 | - |
L-threonine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 1.1 | - |
tRNASer | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 2.433 | - |
DL-Serine hydroxamate | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 2.5 | - |
L-serine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.11 | ATP | - |
Zea mays |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.11 | 0.0027 | - |
L-cysteine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 0.006 | - |
L-threonine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 1.04 | - |
DL-Serine hydroxamate | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 13.9 | - |
L-serine | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays | |
6.1.1.11 | 1275 | - |
tRNASer | in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication | Zea mays |