Literature summary extracted from
Walther, M.; Hofheinz, K.; Vogel, R.; Roffeis, J.; Kuehn, H.
The N-terminal beta-barrel domain of mammalian lipoxygenases including mouse 5-lipoxygenase is not essential for catalytic activity and membrane binding but exhibits regulatory functions (2011), Arch. Biochem. Biophys., 516, 1-9.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.13.11.34 |
phosphatidyl choline |
addition increases catalytic activity of wild-type nzyme by 50% |
Mus musculus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.34 |
expressed in Escherichia coli as a His-tagged fusion protein |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.34 |
DELTA1-114 |
D114 truncation mutant shows 6.8% residual activity compared to wild-type, Km (arachidonate) increased compared to wild-type. Vmax: 9.3% compared to wild-type (100%). Addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties |
Mus musculus |
1.13.11.34 |
DELTA1-125 |
I125 truncation mutant shows 1.8% residual activity compared to wild-type, addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties |
Mus musculus |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.34 |
0.02 |
- |
arachidonate |
wild-type, pH and temperature not specified in the publication |
Mus musculus |
|
1.13.11.34 |
0.06 |
- |
arachidonate |
mutant DELTA1-114, pH and temperature not specified in the publication |
Mus musculus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.34 |
Ca2+ |
addition of Ca2+ increases catalytic activity of wild-type nzyme by 50% |
Mus musculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.34 |
Mus musculus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.11.34 |
using Ni-NTA chromatography |
Mus musculus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.34 |
arachidonate + O2 |
- |
Mus musculus |
(5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.34 |
5-LOX |
- |
Mus musculus |