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Literature summary extracted from

  • Timpson, L.M.; Liliensiek, A.K.; Alsafadi, D.; Cassidy, J.; Sharkeym M.A.; Liddell, S.; Allers, T.; Paradisi, F.
    A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii (2012), Appl. Microbiol. Biotechnol., 97, 195-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 homologously expressed Haloferax volcanii
1.1.1.71 homologously expressed Haloferax volcanii

General Stability

EC Number General Stability Organism
1.1.1.71 most stable when stored at pH 8.0 with 2 M and 3 M KCl Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.071
-
 NADH pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 0.29
-
 NAD+ pH 11.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 28
-
 acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 31
-
 ethanol pH 11.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 0.032
-
 NADPH pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 0.2 1 NADP+ pH 10.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 6.7
-
 1-butanol pH 10.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 9.2
-
 acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 44
-
 ethanol pH 10.0, temperature not specified in the publication Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 KCl optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl and with 1-butanol at pH 10.0 with 4 M KCl. Catalyzes the reductive reaction optimally at pH 6.0 with 4 M KCl Haloferax volcanii
1.1.1.71 KCl maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37600
-
 4 * 37600, SDS-PAGE Haloferax volcanii
1.1.1.1 138000
-
 gel filtration Haloferax volcanii
1.1.1.71 37800
-
 4 * 37800, SDS-PAGE Haloferax volcanii
1.1.1.71 132000
-
 gel filtration Haloferax volcanii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.71 acetonitrile 4°C, 72 h, retains 65% of its original activity following incubation with 5% (v/v) acetonitrile, retains 63% of its original activity following incubation with 10 (v/v) acetonitrile, complete inactivation following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii
1.1.1.71 dimethyl sulfoxide 4°C, 72 h, retains 74% of its original activity following incubation with 5% (v/v) dimethyl sulfoxide, retains 69% of its original activity following incubation with 10 (v/v) dimethyl sulfoxide, retains 47% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii
1.1.1.71 Glycerol stabilizing and cryoprotecting additive for the preservation of the enzyme over a short-time period, 10 days Haloferax volcanii
1.1.1.71 Methanol 4°C, 72 h, retains 70% of its original activity following incubation with 5% (v/v) acetonitrile, retains 67% of its original activity following incubation with 10 (v/v) acetonitrile, retains 39% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Haloferax volcanii D4GSN2
-
-
1.1.1.1 Haloferax volcanii DSM 3757 D4GSN2
-
-
1.1.1.71 Haloferax volcanii D4GP73
-
-
1.1.1.71 Haloferax volcanii DSM 3757 D4GP73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 purified in one step by immobilized metal-affinity chromatography Haloferax volcanii
1.1.1.71 purified in one step by immobilized metal-affinity chromatography Haloferax volcanii

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 -20°C, inactive after approximately 2 weeks, storage at -80°C or lyophilization does not improve storage time Haloferax volcanii
1.1.1.71 -20°C, crude enzyme retains half of its original activity following incubation at -20°C for 75 days and almost one third of its original activity following incubation for 42 days Haloferax volcanii
1.1.1.71 4°C, inactive within 10 days Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-propanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii propanal + NADH + H+
-
 ?
1.1.1.1 1-propanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii DSM 3757 propanal + NADH + H+
-
 ?
1.1.1.1 3-methylbutanal + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii ? + NADH + H+
-
 ?
1.1.1.1 3-methylbutanal + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii DSM 3757 ? + NADH + H+
-
 ?
1.1.1.1 butanol + NAD+ about 15% of the activity with ethanol or 1-propanol Haloferax volcanii 1-butanal + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii acetaldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii DSM 3757 acetaldehyde + NADH + H+
-
 ?
1.1.1.1 additional information the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol Haloferax volcanii ?
-
 ?
1.1.1.1 additional information the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol Haloferax volcanii DSM 3757 ?
-
 ?
1.1.1.1 pentanol + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii pentanal + NADH + H+
-
 ?
1.1.1.1 pentanol + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii DSM 3757 pentanal + NADH + H+
-
 ?
1.1.1.71 1-butanol + NADP+
-
 Haloferax volcanii 1-butanal + NADPH + H+
-
 ?
1.1.1.71 1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii pentanal + NADPH + H+
-
 ?
1.1.1.71 1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii DSM 3757 pentanal + NADPH + H+
-
 ?
1.1.1.71 1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii propanal + NADPH + H+
-
 ?
1.1.1.71 1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii DSM 3757 propanal + NADPH + H+
-
 ?
1.1.1.71 2-butanol + NADP+ about 25% of the activity with 1-butanol Haloferax volcanii butanone + NADPH + H+
-
 ?
1.1.1.71 3-methylbutanal + NADP+ about% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+
-
 ?
1.1.1.71 benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii benzaldehyde + NADPH + H+
-
 ?
1.1.1.71 benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii DSM 3757 benzaldehyde + NADPH + H+
-
 ?
1.1.1.71 ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii acetaldehyde + NADPH + H+
-
 ?
1.1.1.71 ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii DSM 3757 acetaldehyde + NADPH + H+
-
 ?
1.1.1.71 isoamyl alcohol + NADP+ about 80% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+
-
 ?
1.1.1.71 additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii ?
-
 ?
1.1.1.71 additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii DSM 3757 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 37600, SDS-PAGE Haloferax volcanii
1.1.1.71 tetramer 4 * 37800, SDS-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 HvADH1
-
 Haloferax volcanii
1.1.1.1 HVO_2428 locus name Haloferax volcanii
1.1.1.71 HvADH2
-
 Haloferax volcanii
1.1.1.71 HVO_B0071 locus name Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 80
-
-
 Haloferax volcanii
1.1.1.71 85 90
-
 Haloferax volcanii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 60 90 60°C: 75% of maximal activity, 90°C: 75% of maximal activity Haloferax volcanii
1.1.1.71 70 95 70°C: 60% of maximal activity, 95°C: 70% of maximal activity Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
 reduction of aldehyde Haloferax volcanii
1.1.1.1 10
-
 optimally active with 1-butanol at pH 10.0 with 4 M KCl Haloferax volcanii
1.1.1.1 11
-
 optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl Haloferax volcanii
1.1.1.71 6
-
 reduction of aldehyde Haloferax volcanii
1.1.1.71 10
-
 oxidation of alcohol Haloferax volcanii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.71 8
-
 most stable when stored at pH 8.0 with 2 and 3 M KCl Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ exclusively NAD+ dependent Haloferax volcanii
1.1.1.71 NAD+ dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl Haloferax volcanii
1.1.1.71 NADP+ dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl Haloferax volcanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 0.048
-
 acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 0.072
-
 ethanol pH 11.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 9.037
-
 NAD+ pH 11.0, temperature not specified in the publication Haloferax volcanii
1.1.1.1 32.77
-
 NADH pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 0.07
-
 ethanol pH 10.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 0.479
-
 1-butanol pH 10.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 0.744
-
 acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 12.39
-
 NADP+ pH 10.0, temperature not specified in the publication Haloferax volcanii
1.1.1.71 242.8
-
 NADPH pH 6.0, temperature not specified in the publication Haloferax volcanii