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Literature summary extracted from

  • Liang, M.; Chen, M.; Liu, X.; Zhai, Y.; Liu, X.W.; Zhang, H.; Xiao, M.; Wang, P.
    Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation (2012), Appl. Microbiol. Biotechnol., 93, 1469-1474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Escherichia coli BL21(DE3) cells Thermoanaerobacter mathranii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 Cu2+ 23% residual activity at 1 mM Thermoanaerobacter mathranii
5.3.1.4 Ni2+ 85% residual activity at 1 mM Thermoanaerobacter mathranii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ improves the enzymatic activity to 121% at 5 mM Thermoanaerobacter mathranii
5.3.1.4 Mn2+ highest activity with Mn2+. The optimum Mn2+ concentration for the free and calcium alginate-immobilized enzyme is 5 mM (132% activity) Thermoanaerobacter mathranii
5.3.1.4 additional information not activated by Mg2+, Fe2+, and Ca2+ Thermoanaerobacter mathranii
5.3.1.4 Zn2+ improves the enzymatic activity to 115% at 5 mM Thermoanaerobacter mathranii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 54000
-
 x * 54000, SDS-PAGE Thermoanaerobacter mathranii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Thermoanaerobacter mathranii
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Thermoanaerobacter mathranii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 Ni-NTA column chromatography Thermoanaerobacter mathranii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Thermoanaerobacter mathranii D-tagatose 43.9% yield with immobilized enzyme ?
5.3.1.4 L-arabinose
-
 Thermoanaerobacter mathranii L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 54000, SDS-PAGE Thermoanaerobacter mathranii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 60
-
 free enzyme Thermoanaerobacter mathranii
5.3.1.4 75
-
 calcium alginate-immobilized enzyme Thermoanaerobacter mathranii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7.5
-
 calcium alginate-immobilized enzyme Thermoanaerobacter mathranii
5.3.1.4 8
-
 free enzyme Thermoanaerobacter mathranii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 6.5 9 the activity of the free enzyme remains about 80% between pH 6.5 and 9.0, while the activity remains above 80% between pH 5.5 and 8.0 in case of the immobilized enzyme Thermoanaerobacter mathranii