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Literature summary extracted from

  • Zhang, W.; Chen, L.; Liu, D.
    Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction (2012), Appl. Microbiol. Biotechnol., 93, 1305-1314.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.16.1.1 environmental protection the organism can potentially be used for bioremediation in marine environments Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.1.1 gene merA, mer operon located on Tn5041, DNA and amino acid sequence determination and analysis, chromosomal localization, real-time PCR enzyme expression analysis Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.16.1.1 additional information the organism is highly resistant to the antibiotics ampicillin, kanamycin, chloramphenicol, and tetracycline Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.16.1.1 additional information the organism is resistant to CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, ZnSO4, and to 0.28 mM HgCl2 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+ Pseudomonas putida the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+ Pseudomonas putida SP1 the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to Hg2+ + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.1 Pseudomonas putida
-
 marine-isolated mercury-resistant strain, isolated from seawater collected from Yantai coastal zone in Shandong Province, China, gene merA encoded in the mer operon
-
1.16.1.1 Pseudomonas putida SP1
-
 marine-isolated mercury-resistant strain, isolated from seawater collected from Yantai coastal zone in Shandong Province, China, gene merA encoded in the mer operon
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.1.1 additional information the low virulence strain is part of the biofilm. Optimal pH for the growth and enzyme activity of strain SP1 in presence of HgCl2 is pH 8.0-9.0, whereas optimal pH for expression of merA is pH 5.0 Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.1 Hg + NADP+ + H+
-
 Pseudomonas putida Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+ the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to Pseudomonas putida Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+
-
 Pseudomonas putida SP1 Hg2+ + NADPH
-
 r
1.16.1.1 Hg + NADP+ + H+ the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to Pseudomonas putida SP1 Hg2+ + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.16.1.1 mercuric reductase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.16.1.1 28
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.1.1 8
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.16.1.1 5 9 high activity within that range Pseudomonas putida