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Literature summary extracted from

  • Schütte, W.; Hummel, W.; Kula, M.R.
    L-2-Hydroxyisocaproate dehydrogenase - A new enzyme from Lactobacillus confusus for the stereospecific reduction of 2-ketocarboxylic acids (1984), Appl. Microbiol. Biotechnol., 19, 167-176.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.337 1,10-phenanthroline 10 mM, 15% inhibition Weissella confusa
1.1.1.337 4-chloromercuribenzoate weak inhibition Weissella confusa
1.1.1.337 iodoacetamide weak inhibition Weissella confusa
1.1.1.337 iodoacetate weak inhibition Weissella confusa
1.1.1.337 KCN weak inhibition Weissella confusa
1.1.1.337 additional information reduced glutathione (20 mM) has no effect. EDTA, 2,2'-bipyridyl in 1 mM and 10 mM final concentrations have no effect on enzymatic activity Weissella confusa
1.1.1.337 NADH inhibition above 0.24 mM Weissella confusa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.337 0.06
-
 2-oxoisocaproate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.065
-
 2-oxoisovalerate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.1
-
 2-oxovalerate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.1 2 phenylpyruvate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.1 2 2-oxocaproate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.17
-
 2-oxooctanoate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.45
-
 2-oxobutyrate pH 7.0, 30°C Weissella confusa
1.1.1.337 0.6
-
 2-Hydroxyisovalerate pH 8.0, 30°C Weissella confusa
1.1.1.337 0.62
-
 2-hydroxyisocaproate pH 8.0, 30°C Weissella confusa
1.1.1.337 0.64
-
 3-Phenyllactate pH 8.0, 30°C Weissella confusa
1.1.1.337 1.8
-
 2-hydroxyvalerate pH 8.0, 30°C Weissella confusa
1.1.1.337 1.9
-
 2-Hydroxyoctanoate pH 8.0, 30°C Weissella confusa
1.1.1.337 2.2
-
 2-hydroxycaproate pH 8.0, 30°C Weissella confusa
1.1.1.337 4.5
-
 pyruvate pH 7.0, 30°C Weissella confusa
1.1.1.337 100
-
 L-lactate pH 8.0, 30°C Weissella confusa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.337 additional information at 1 mM no influence is observed with Mg2+, Ca2+, Co2+, Ni2+, and Cd2+ and very weak effects with Mn2+, Cu2+, and Zn2+ Weissella confusa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.337 33000
-
 4 * 33000, SDS-PAGE Weissella confusa
1.1.1.337 125000
-
 gel filtration Weissella confusa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.337 a 2-oxocarboxylate + NADH + H+ Weissella confusa
-
 (2S)-2-hydroxycarboxylate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.337 Weissella confusa P14295 i.e. Lactobacillus confusus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.337
-
 Weissella confusa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.337 culture condition maximum specific and volume activities are found at the end of the exponential growth phase, during further fermentation enzyme activity declines rapidly and is no longer detectable after 15 h Weissella confusa
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.337 4°C, pH 6.5, concentrated enzyme solution, 10% loss of activity after 6 months Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.337 2-hydroxycaproate + NAD+
-
 Weissella confusa 2-oxocaproate + NADH + H+
-
 r
1.1.1.337 2-hydroxyisocaproate + NAD+ the initial rate of the reduction of 2-oxoisocaproate is about seven times faster than the reverse reaction, the dehydrogenation of L-2-hydroxyisocaproate, measured in buffers at the respective pH optima Weissella confusa 2-oxoisocaproate + NADH + H+
-
 r
1.1.1.337 2-hydroxyisovalerate + NAD+
-
 Weissella confusa 2-oxoisovalerate + NADH + H+
-
 r
1.1.1.337 2-hydroxyoctanoate + NAD+
-
 Weissella confusa 2-oxooctanoate + NADH + H+
-
 ?
1.1.1.337 2-hydroxyvalerate + NAD+
-
 Weissella confusa 2-oxovalerate + NADH + H+
-
 r
1.1.1.337 2-oxobutyrate + NADH + H+
-
 Weissella confusa 2-hydroxybutyrate + NAD+
-
 r
1.1.1.337 2-oxocaproate + NADH + H+
-
 Weissella confusa 2-hydroxycaproate + NAD+
-
 r
1.1.1.337 2-oxoisocaproate + NADH + H+ 2-oxoisocaproate is the best substrate with the lowest KM-valus of 0.065 mM. Tthe initial rate of the reduction of 2-oxoisocaproate is about seven times faster than the reverse reaction, the dehydrogenation of L-2-hydroxyisocaproate, measured in buffers at the respective pH optima Weissella confusa 2-hydroxyisocaproate + NAD+
-
 r
1.1.1.337 2-oxoisovalerate + NADH + H+
-
 Weissella confusa 2-hydroxyisovalerate + NAD+
-
 r
1.1.1.337 2-oxooctanoate + NADH + H+
-
 Weissella confusa 2-hydroxyoctanoate + NAD+
-
 r
1.1.1.337 2-oxovalerate + NADH + H+
-
 Weissella confusa 2-hydroxyvalerate + NAD+
-
 r
1.1.1.337 3-phenyllactate + NAD+
-
 Weissella confusa pyruvate + NADH + H+
-
 r
1.1.1.337 a 2-oxocarboxylate + NADH + H+
-
 Weissella confusa (2S)-2-hydroxycarboxylate + NAD+
-
 r
1.1.1.337 a 2-oxocarboxylate + NADH + H+ various 2-oxocarboxylic acids are stereospecifically reduced to the corresponding (S)-2-hydroxycarboxylic acids. In the reverse reaction the NAD+-dependent dehydrogenation of L-2-hydroxycarboxylic acids is observed Weissella confusa (2S)-2-hydroxycarboxylate + NAD+
-
 r
1.1.1.337 L-lactate + NAD+
-
 Weissella confusa pyruvate + NADH + H+
-
 r
1.1.1.337 phenylpyruvate + NADH + H+
-
 Weissella confusa 3-phenyllactate + NAD+
-
 r
1.1.1.337 pyruvate + NADH + H+
-
 Weissella confusa L-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.337 tetramer 4 * 33000, SDS-PAGE Weissella confusa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.337 L-2-hydroxyisocaproate dehydrogenase
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.337 30
-
 assay at Weissella confusa
1.1.1.337 50
-
-
 Weissella confusa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.337 30 50 the maximal temperature is around 50°C. At this temperature the reaction proceeded at about 3fold increase of the rate compared to 30°C Weissella confusa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.337 40
-
 1 h, stable up to Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.337 7
-
 reduction of 2-oxoisocaproate Weissella confusa
1.1.1.337 8 8.5 dehydrogenation of L-2-hydroxyisocaproate Weissella confusa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.337 5.5 9 4°C, 24 h, stable Weissella confusa
1.1.1.337 6.5
-
 4°C, 6 months, concentrated enzyme solutions, 10% loss of activity Weissella confusa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.337 NAD+ the enzyme requires NAD+ as a cofactor, no reaction with NADP+ in concentrations up to 3 mM Weissella confusa
1.1.1.337 NADH the enzyme requires NADH as a cofactor, no reaction with NADPH up to 0.24 mM Weissella confusa