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Literature summary extracted from

  • Repizo, G.D.; Blancato, V.S.; Mortera, P.; Lolkema, J.S.; Magni, C.
    Biochemical and genetic characterization of the Enterococcus faecalis oxaloacetate decarboxylase complex (2013), Appl. Environ. Microbiol., 79, 2882-2890.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.112 expression of gene oadH as maltose-binding protein fusion protein in Escherichia coli, genes oadA-oadD, expression of recombinant His-tagged subunits in Escherichia coli strain BL21(DE3) Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.112 additional information oadA, oadB, and oadD deletion mutants are constructed, while an oadH mutant strain cannot be obtained in spite of using different genetic strategies Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.112 cytoplasm subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane Enterococcus faecalis 5737
-
4.1.1.112 membrane Ef-B is a membrane-bound subunit, while subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane Enterococcus faecalis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.112 Oxaloacetate Enterococcus faecalis
-
 Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate Enterococcus faecalis JH2-2
-
 Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Enterococcus faecalis
-
 genes oadA, oadB, oadC, and oadD
-
4.1.1.112 Enterococcus faecalis JH2-2
-
 genes oadA, oadB, oadC, and oadD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112 recombinnat maltose-binding protein fusion protein OadH from Escherichia coli by amylase affinity chromatography, recombinant His-tagged subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 Oxaloacetate
-
 Enterococcus faecalis Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate
-
 Enterococcus faecalis JH2-2 Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.112 tetramer the enzyme is constituted of four subunits: catalytic subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and subunit Ef-H. Subunits Ef-A, Ef-D, and Ef-H form a cytoplasmic soluble complex, Ef-AHD, which is also associated with the membrane. The Ef-H subunit is involved in the cytoplasmic Ef-AHD complex formation. Analysis of subunit interactions and complex formation, overview Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.112 OAD
-
 Enterococcus faecalis

General Information

EC Number General Information Comment Organism
4.1.1.112 physiological function presence of the membrane-bound Ef-B subunit is required for full alkalinization of the internal medium of Enterococcus faecalis cells during citrate fermentation Enterococcus faecalis