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Literature summary extracted from
- Nitrate-dependent degradation of acetone by Alicycliphilus and Paracoccus strains and comparison of acetone carboxylase enzymes (2011), Appl. Environ. Microbiol., 77, 6821-6825.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | additional information | - |
three bands observed with SDS-PAGE: 85000, 78000, 20000 Da | Alicycliphilus denitrificans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.6 | acetone + CO2 + ATP + H2O | Alicycliphilus denitrificans | - |
acetoacetate + AMP + phosphate | - |
? |  |
| 6.4.1.6 | butanone + CO2 + ATP + H2O | Alicycliphilus denitrificans | strain KN Bun08 also carboxylates also butanone | 3-oxopentanoate + AMP + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.4.1.6 | Alicycliphilus denitrificans | - |
isolated from strain KN Bun08 and K601T | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.4.1.6 | enriched from cell extracts of Alicycliphilus denitrificans by two subsequent DEAE Sepharose column procedures | Alicycliphilus denitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.4.1.6 | acetone + CO2 + ATP + H2O | - |
Alicycliphilus denitrificans | acetoacetate + AMP + phosphate | - |
? | |
| 6.4.1.6 | butanone + CO2 + ATP + H2O | strain KN Bun08 also carboxylates also butanone | Alicycliphilus denitrificans | 3-oxopentanoate + AMP + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.4.1.6 | acetone-carboxylating enzyme | - |
Alicycliphilus denitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | 28 | - |
assay at | Alicycliphilus denitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.4.1.6 | 8 | - |
assay at | Alicycliphilus denitrificans |
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Release 2023.1 (January 2023)
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