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Literature summary extracted from

  • Stekhanova, T.N.; Mardanov, A.V.; Bezsudnova, E.Y.; Gumerov, V.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.
    Characterization of a thermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus (2010), Appl. Environ. Microbiol., 76, 4096-4098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 expression in Escherichia coli Thermococcus sibiricus
1.1.1.71 expressed in Escherichia coli Thermococcus sibiricus
1.1.1.71 expression in Escherichia coli Thermococcus sibiricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 EDTA 5 mM, 80% of initial activity Thermococcus sibiricus
1.1.1.2 Fe2+ 10 mM, 74% of initial activity Thermococcus sibiricus
1.1.1.2 Mg2+ 10 mM, 78% of initial activity Thermococcus sibiricus
1.1.1.2 Zn2+ 10 mM, 79% of initial activity Thermococcus sibiricus
1.1.1.71 acetonitrile complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 chloroform 21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 Dimethyl formamide 87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 dimethyl sulfoxide complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 EDTA 5 mM, 20% inhibition; 5 mM, 80% of initial activity Thermococcus sibiricus
1.1.1.71 ethyl acetate complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 Fe2+ 10 mM, 74% of initial activity Thermococcus sibiricus
1.1.1.71 FeSO4 10 mM, 26% inhibition Thermococcus sibiricus
1.1.1.71 methanol 75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 Mg2+ 10 mM, 78% of initial activity Thermococcus sibiricus
1.1.1.71 n-decane 9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 n-hexane 40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl Thermococcus sibiricus
1.1.1.71 Zn2+ 10 mM, 79% of initial activity Thermococcus sibiricus
1.1.1.71 ZnSO4 10 mM, 21% inhibition Thermococcus sibiricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.02
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
1.1.1.2 0.022
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
1.1.1.2 17.75
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
1.1.1.2 54.4
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.1.1.2 168
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 0.02
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
1.1.1.71 0.02
-
 NADPH 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 0.022
-
 NADP+ 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.022
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 17.75
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
1.1.1.71 17.75
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 54.4
-
 D-xylose 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 54.4
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 168
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 168
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.2 KCl 600 mM, 147% of initial activity Thermococcus sibiricus
1.1.1.2 NaCl 400 mM, 206% of initial activity Thermococcus sibiricus
1.1.1.71 KCl activity increases in the presence of KCl is maintained even at concentration of 3 M Thermococcus sibiricus
1.1.1.71 KCl 600 mM, 147% of initial activity Thermococcus sibiricus
1.1.1.71 additional information metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA Thermococcus sibiricus
1.1.1.71 NaCl activity increases in the presence of NaCl is maintained even at concentration of 4 M Thermococcus sibiricus
1.1.1.71 NaCl 400 mM, 206% of initial activity Thermococcus sibiricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.71 26200
-
 2 * 26200, calculated from sequence Thermococcus sibiricus
1.1.1.71 28700
-
 2 * 28700, SDS-PAGE Thermococcus sibiricus
1.1.1.71 56000 60000 gel filtration Thermococcus sibiricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Thermococcus sibiricus C6A190
-
-
1.1.1.2 Thermococcus sibiricus DSM 12597 C6A190
-
-
1.1.1.71 Thermococcus sibiricus C6A190
-
-
1.1.1.71 Thermococcus sibiricus DSM 12597 C6A190
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71
-
 Thermococcus sibiricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (S)-2-butanol + NADP+ 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol Thermococcus sibiricus butanone + NADPH + H+
-
 ?
1.1.1.2 2-pentanol + NADP+ 67% of the activity with 2-propanol Thermococcus sibiricus pentan-2-one + NADPH + H+
-
 ?
1.1.1.2 2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH + H+
-
 ?
1.1.1.2 2-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 acetone + NADPH + H+
-
 ?
1.1.1.2 benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
 ?
1.1.1.2 benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 benzaldehyde + NADPH + H+
-
 ?
1.1.1.2 cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.2 cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 ? + NADPH + H+
-
 ?
1.1.1.2 D-arabinose + NADP+ 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose Thermococcus sibiricus D-arabitol + NADPH + H+
-
 ?
1.1.1.2 D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.2 D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus DSM 12597 ? + NADPH + H+
-
 ?
1.1.1.2 D-xylose + NADP+ 246% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.2 dimethylglyoxal + NADPH + H+ 270% of the activity with pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.2 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus lactaldehyde + NADP+
-
 ?
1.1.1.2 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus DSM 12597 lactaldehyde + NADP+
-
 ?
1.1.1.71 (S)-2-butanol + NADP+ 196% relative activity as compared to 2-propanol Thermococcus sibiricus butanone + NADPH
-
 ?
1.1.1.71 (S)-2-butanol + NADP+ 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol Thermococcus sibiricus butanone + NADPH + H+
-
 ?
1.1.1.71 1,3-butanediol + NADP+ 91% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 1-butanol + NADP+ 80% relative activity as compared to 2-propanol Thermococcus sibiricus butanal + NADPH
-
 ?
1.1.1.71 1-phenylmethanol + NADP+ 180% relative activity as compared to 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
 ?
1.1.1.71 2-pentanol + NADP+ 67% relative activity as compared to 2-propanol Thermococcus sibiricus 2-pentanone + NADPH + H+
-
 ?
1.1.1.71 2-pentanol + NADP+ 67% of the activity with 2-propanol Thermococcus sibiricus pentan-2-one + NADPH + H+
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH + H+
-
 ?
1.1.1.71 3-methyl-2-pentanone + NADPH + H+ 13% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.71 benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+
-
 ?
1.1.1.71 cellobiose + NADP+ 71% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-arabinose + NADP+ 200% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-arabinose + NADP+ 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose Thermococcus sibiricus D-arabitol + NADPH + H+
-
 ?
1.1.1.71 D-glucose + NADP+ 146% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-mannose + NADP+ 48% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-ribose + NADP+ 35% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-xylose + NADP+ 246% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 D-xylose + NADP+ 246% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 dimethylglyoxal + NADPH + H+ 270% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.71 dimethylglyoxal + NADPH + H+ 270% of the activity with pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.71 ethanol + NADP+ 36% relative activity as compared to 2-propanol Thermococcus sibiricus acetaldehyde + NADPH + H+
-
 ?
1.1.1.71 glycerol + NADP+ 16% relative activity as compared to 2-propanol Thermococcus sibiricus glyceraldehyde + NADPH + H+
-
 ?
1.1.1.71 glyoxylic acid + NADPH + H+ 36% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+
-
 ?
1.1.1.71 L-arabinose + NADP+ 17% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+
-
 ?
1.1.1.71 additional information no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose Thermococcus sibiricus ?
-
 ?
1.1.1.71 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus lactaldehyde + NADP+
-
 ?
1.1.1.71 pyruvaldehyde + NADPH + H+
-
 Thermococcus sibiricus ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.71 dimer 2 * 26200, calculated from sequence Thermococcus sibiricus
1.1.1.71 dimer 2 * 28700, SDS-PAGE Thermococcus sibiricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 Tsib_0319 locus name Thermococcus sibiricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.71 60
-
 assay at Thermococcus sibiricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 70
-
 1.5 h, no loss of activity Thermococcus sibiricus
1.1.1.2 80
-
 1.5 h, no loss of activity Thermococcus sibiricus
1.1.1.2 90
-
 half-life 2 h Thermococcus sibiricus
1.1.1.2 100
-
 half-life 1 h Thermococcus sibiricus
1.1.1.71 70
-
 2 h, 10% loss of activity Thermococcus sibiricus
1.1.1.71 70
-
 1.5 h, no loss of activity Thermococcus sibiricus
1.1.1.71 80
-
 2 h, 15% loss of activity Thermococcus sibiricus
1.1.1.71 80
-
 1.5 h, no loss of activity Thermococcus sibiricus
1.1.1.71 90
-
 half-life: 2 h Thermococcus sibiricus
1.1.1.71 90
-
 half-life 2 h Thermococcus sibiricus
1.1.1.71 100
-
 half-life: 1 h Thermococcus sibiricus
1.1.1.71 100
-
 half-life 1 h Thermococcus sibiricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 0.45
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
1.1.1.2 0.53
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus
1.1.1.2 0.7
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.1.1.2 1.51
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
1.1.1.2 2.04
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
1.1.1.71 0.45
-
 NADP+ 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.45
-
 NADP+ pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 0.53
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.53
-
 2-propanol pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 0.7
-
 D-xylose 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.7
-
 D-xylose pH 10.5, 60°C Thermococcus sibiricus
1.1.1.71 1.51
-
 NADPH pH 7.5, 60°C Thermococcus sibiricus
1.1.1.71 1.51
-
 NADPH 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 2.04
-
 Pyruvaldehyde pH 7.5, 60°C Thermococcus sibiricus
1.1.1.71 2.04
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7.5
-
 assay at, reduction of ketones Thermococcus sibiricus
1.1.1.2 10.5
-
 assay at, oxidation of alcohols Thermococcus sibiricus
1.1.1.71 7.5
-
 assay at, reduction of ketones Thermococcus sibiricus
1.1.1.71 10.5
-
 assay at, oxidation of alcohols Thermococcus sibiricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+
-
 Thermococcus sibiricus
1.1.1.2 NADPH
-
 Thermococcus sibiricus
1.1.1.71 NADPH
-
 Thermococcus sibiricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.71 0.003
-
 2-propanol 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.013
-
 D-xylose 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 0.11
-
 Pyruvaldehyde 60°C, pH 7.5 Thermococcus sibiricus
1.1.1.71 20.45
-
 NADP+ 60°C, pH 10.5 Thermococcus sibiricus
1.1.1.71 75.5
-
 NADPH 60°C, pH 7.5 Thermococcus sibiricus