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Literature summary extracted from
- Enzymatic analysis of uridine diphosphate N-acetyl-D-glucosamine (2008), Anal. Biochem., 381, 94-100.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.1.374 | analysis | high-throughput chromatographic analysis of UDPGlcNAc in a complex matrix of deproteinized cell extract | Methanococcus maripaludis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.374 | expression in Escherichia coli BL21(DE3) | Methanococcus maripaludis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.374 | additional information | N-acetyl-D-glucosamine, D-glucose, and D-glucosamine do not inhibit the reaction at 3-mM concentrations | Methanococcus maripaludis | |
| 1.1.1.374 | UDP | 2 mM, reduces activity by 50% | Methanococcus maripaludis |  |
| 1.1.1.374 | UDP-alpha-D-glucose | 2 mM, reduces activity by 50% | Methanococcus maripaludis | |
| 1.1.1.374 | UDP-alpha-N-acetyl-D-galactosamine | 3 mM, reduces activity by 50% | Methanococcus maripaludis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.374 | 0.18 | - |
NAD+ | pH 8.5, 37°C | Methanococcus maripaludis | |
| 1.1.1.374 | 0.18 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 8.5, 37°C | Methanococcus maripaludis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.374 | K+ | activity is 85% lower in the absence of KCl as compared with reactions containing 200 mM KCl | Methanococcus maripaludis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.374 | 39000 | - |
8 * 39000, SDS-PAGE | Methanococcus maripaludis |
| 1.1.1.374 | 305000 | - |
gel filtration | Methanococcus maripaludis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.374 | UDP-N-acetyl-alpha-D-glucosamine + NAD+ | Methanococcus maripaludis | the enzyme is involved in the biosynthesis of glycan component sugars | UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.374 | Methanococcus maripaludis | Q6M0B9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.374 | - |
Methanococcus maripaludis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.374 | -80°C, stable for at least several weeks in 20% glycerol | Methanococcus maripaludis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.374 | UDP-N-acetyl-alpha-D-glucosamine + NAD+ | the enzyme is involved in the biosynthesis of glycan component sugars | Methanococcus maripaludis | UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+ | - |
? | |
| 1.1.1.374 | UDP-N-acetyl-alpha-D-glucosamine + NAD+ | no activity is detected when NAD+ is replaced with NADP+. The MMP0352 protein does not catalyze the oxidation of the substrate analogs UDPGlc, UDPGalNAc, N-acetylglucosamine, and glucosamine | Methanococcus maripaludis | UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.374 | octamer | 8 * 39000, SDS-PAGE | Methanococcus maripaludis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.374 | MMP0352 | locus name | Methanococcus maripaludis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.374 | 37 | - |
assay at | Methanococcus maripaludis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.374 | 0.9 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 8.5, 37°C | Methanococcus maripaludis | |
| 1.1.1.374 | 0.99 | - |
NAD+ | pH 8.5, 37°C | Methanococcus maripaludis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.374 | 8.5 | - |
assay at | Methanococcus maripaludis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.374 | physiological function | the enzyme is involved in the biosynthesis of glycan component sugars | Methanococcus maripaludis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.374 | 5 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 8.5, 37°C | Methanococcus maripaludis | |
| 1.1.1.374 | 5.5 | - |
NAD+ | pH 8.5, 37°C | Methanococcus maripaludis | |
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Release 2023.1 (January 2023)
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