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Literature summary extracted from

  • Hara, R.; Kino, K.
    Enhanced synthesis of 5-hydroxy-L-tryptophan through tetrahydropterin regeneration (2013), AMB Express, 3, 70.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.1 gene tnaA Escherichia coli
4.2.1.96 expression in recombinant Escherichia coli strain BL21(DE3) tnaA-, co-expression with Chromobacterium violaceum L-phenylalanine 4-hydroxylase mutant L101Y-W180F, Escherichia coli dihydropteridine reductase, and Bacillus subtilis glucose dehydrogenase genes for installation of 5-hydroxy-L-tryptophan biosynthesis in the recombinant Escherichia coli strain, method optimization and development of a cofactor regeneration system for L-tryptophan hydroxylation for enhanced synthesis of 5-hydroxy-L-tryptophan Pseudomonas syringae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.1 additional information tnaA gene disruption of tryptophanase in Escherichia coli strain BL21(DE3) to prevent L-tryptophan and 5-hydroxy-L-tryptophan degradation for enhanced whole cell synthesis of 5-hydroxy-L-tryptophan using modified L-phenylalanine 4-hydroxylase, PAH-L101Y-W180F, from Chromobacterium violaceum in Escherichia coli, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.1 L-tryptophan + H2O Escherichia coli
-
 indole + pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Escherichia coli
-
 gene tnaA
-
4.2.1.96 Pseudomonas syringae Q48G05
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-tryptophan + H2O
-
 Escherichia coli indole + pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.96 PCD
-
 Pseudomonas syringae
4.2.1.96 Pterin-4alpha-carbinolamine dehydratase
-
 Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.1 7.5
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
4.1.99.1 metabolism tryptophanase is an initial enzyme for the degradation of L-tryptophan and 5-hydroxytryptophan in Escherichia coli Escherichia coli