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Literature summary extracted from

  • Blundell, R.D.; Williams, S.J.; Morrow, C.A.; Ericsson, D.J.; Kobe, B.; Fraser, J.A.
    Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans (2013), Acta Crystallogr. Sect. F, 69, 1033-1036.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4 expressed in Escherichia coli BL21(DE3)pLysS cells Cryptococcus neoformans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.4 hanging drop vapor diffusion method, using 0.1 M bis-Tris propane pH 8, 0.2 M sodium bromide, 17% (w/v) polyethylene glycol 3350 Cryptococcus neoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.4 Mg2+ required Cryptococcus neoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 46000
-
 2 * 46000, SDS-PAGE Cryptococcus neoformans

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Cryptococcus neoformans
-
 Cryptococcus neoformans variant grubii
-
6.3.4.4 Cryptococcus neoformans H99
-
 Cryptococcus neoformans variant grubii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4 HisTrap column chromatography and Superdex 200 gel filtration Cryptococcus neoformans

Subunits

EC Number Subunits Comment Organism
6.3.4.4 homodimer 2 * 46000, SDS-PAGE Cryptococcus neoformans

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 Adenylosuccinate synthetase
-
 Cryptococcus neoformans
6.3.4.4 AdSS
-
 Cryptococcus neoformans

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.4 GTP
-
 Cryptococcus neoformans