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Literature summary extracted from

  • Lyashenko, A.V.; Bezsudnova, E.Y.; Gumerov, V.M.; Lashkov, A.A.; Mardanov, A.V.; Mikhailov, A.M.; Polyakov, K.M.; Popov, V.O.; Ravin, N.V.; Skryabin, K.G.; Zabolotniy, V.K.; Stekhanova, T.N.; Kovalchuk, M.V.
    Expression, purification and crystallization of a thermostable short-chain alcohol dehydrogenase from the archaeon Thermococcus sibiricus (2010), Acta Crystallogr. Sect. F, 66, 655-657.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 expression in Escherichia coli Thermococcus sibiricus
1.1.1.71 expression in Escherichia coli Thermococcus sibiricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.2 to 1.68 A resolution, crystals belong to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A Thermococcus sibiricus
1.1.1.71 hanging-drop vapour-diffusion method using 25% (w/v) polyethylene glycol 3350 pH 7.5 as precipitant Thermococcus sibiricus
1.1.1.71 to 1.68 A resolution, crystals belong to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A Thermococcus sibiricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Thermococcus sibiricus C6A190
-
-
1.1.1.2 Thermococcus sibiricus DSM 12597 C6A190
-
-
1.1.1.71 Thermococcus sibiricus C6A190
-
-
1.1.1.71 Thermococcus sibiricus DSM 12597 C6A190
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71
-
 Thermococcus sibiricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1-propanol + NADP+
-
 Thermococcus sibiricus propanal + NADPH + H+
-
 ?
1.1.1.2 1-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 propanal + NADPH + H+
-
 ?
1.1.1.71 1-propanol + NADP+
-
 Thermococcus sibiricus propanal + NADPH + H+
-
 ?
1.1.1.71 1-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 propanal + NADPH + H+
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus acetone + NADPH
-
 ?
1.1.1.71 2-propanol + NADP+
-
 Thermococcus sibiricus DSM 12597 acetone + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 Adh319
-
 Thermococcus sibiricus
1.1.1.71 Adh319
-
 Thermococcus sibiricus
1.1.1.71 Tsib_0319 locus name Thermococcus sibiricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 60
-
 assay at Thermococcus sibiricus
1.1.1.71 60
-
 assay at Thermococcus sibiricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADP+
-
 Thermococcus sibiricus