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Literature summary extracted from

  • Connor, S.E.; Capodagli, G.C.; Deaton, M.K.; Pegan, S.D.
    Structural and functional characterization of Mycobacterium tuberculosis triosephosphate isomerase (2011), Acta Crystallogr. Sect. D, 67, 1017-1022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.1 hanging drop vapor diffusion method, using 16% (w/v) PEG 3350 and 250 mM ammonium citrate Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.0025
-
 dihydroxyacetone phosphate in 100 mM Tris pH 7.8, at 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Mycobacterium tuberculosis P9WG43
-
-
5.3.1.1 Mycobacterium tuberculosis H37Rv P9WG43
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 Ni-NTA His-Bind resin column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 dihydroxyacetone phosphate
-
 Mycobacterium tuberculosis D-glyceraldehyde 3-phosphate
-
 ?
5.3.1.1 dihydroxyacetone phosphate
-
 Mycobacterium tuberculosis H37Rv D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TpI
-
 Mycobacterium tuberculosis
5.3.1.1 Triosephosphate isomerase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 730
-
 dihydroxyacetone phosphate in 100 mM Tris pH 7.8, at 25°C Mycobacterium tuberculosis