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Literature summary extracted from
- Crystallization and preliminary X-ray diffraction analysis of L-aminoacylase from the hyperthermophilic archaeon Thermococcus litoralis (2002), Acta Crystallogr. Sect. D, 58, 507-510.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | the enzyme is crystallized from ammonium sulfate using the sitting-drop vapour-diffusion method. The crystals diffract to 2.8 A resolution and belong to the rhombohedral space group R32, with unit-cell parameters a = b = 102.4, c = 178.5 A, gamma = 120° in a hexagonal lattice setting. The asymmetric unit contains one enzyme monomer, containing a single zinc ion | Thermococcus litoralis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.14 | Zn2+ | contains one Zn2+ per monomer, zinc-containing enzyme | Thermococcus litoralis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.14 | 43000 | - |
4 * 43000, SDS-PAGE | Thermococcus litoralis |
| 3.5.1.14 | 170000 | - |
gel filtration | Thermococcus litoralis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.14 | Thermococcus litoralis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | - |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.14 | 0.0092 | - |
pH 8.0, temperature not specified in the publication, substrate: acetyl-DL-Trp | Thermococcus litoralis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.14 | N-acetyl-DL-Trp + H2O | - |
Thermococcus litoralis | acetate + DL-Trp | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.14 | homotetramer | 4 * 43000, SDS-PAGE | Thermococcus litoralis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.14 | 8 | - |
assay at | Thermococcus litoralis |
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