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Literature summary extracted from

  • Dai, C.; Liu, L.; Wang, M.H.
    Characterization of a methionine sulfoxide reductase B from tomato (Solanum lycopersicum), and its protecting role in Saccharomyces cerevisiae (2013), Protein J., 32, 39-47.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 expressed in Escherichia coli BL21 (DE3)cells and in Saccharomyces cerevisiae strain BY4741 Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 C181S inactive Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.12 0.0091
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.12 chloroplast
-
 Solanum lycopersicum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.12 21200
-
 x * 21200, calculated from amino acid sequence Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 Ni2+-affinity column chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.12 flower high expression Solanum lycopersicum
-
1.8.4.12 fruit high expression in red mature fruits Solanum lycopersicum
-
1.8.4.12 leaf high expression Solanum lycopersicum
-
1.8.4.12 root low expression Solanum lycopersicum
-
1.8.4.12 stem low expression Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 L-methionine (R)-sulfoxide + dithiothreitol
-
 Solanum lycopersicum L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 additional information isoform MSRB1 exhibits no activity in thioredoxin dependent system Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.12 ? x * 21200, calculated from amino acid sequence Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase B
-
 Solanum lycopersicum
1.8.4.12 MsrB
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.12 0.09
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.4.12 Solanum lycopersicum calculated from amino acid sequence
-
 8.9

General Information

EC Number General Information Comment Organism
1.8.4.12 physiological function the MSRB1 gene plays a critical role in protecting against oxidative stress Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.12 10
-
 dithiothreitol recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C Solanum lycopersicum