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Literature summary extracted from

  • Hevener, K.E.; Mehboob, S.; Boci, T.; Truong, K.; Santarsiero, B.D.; Johnson, M.E.
    Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis (2012), Protein Expr. Purif., 85, 100-108.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.10 NH4Cl required for stability and activity. Assay in presence of 0.1 M NH4Cl Porphyromonas gingivalis

Application

EC Number Application Comment Organism
1.3.1.39 analysis sensitive assay method measuring the fluorescence decrease of NADPH as it is converted to NADP+ during the reaction, optimized for high-throughput screening Porphyromonas gingivalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.10 expression in Escherichia coli Porphyromonas gingivalis
1.3.1.39 expression in Escherichia coli Porphyromonas gingivalis

General Stability

EC Number General Stability Organism
1.3.1.10 enzyme can be concentrated to 20 mg/ml without precipitation Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.10 NADPH
-
 Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.10 0.0171
-
 NADPH pH 7.5, 25°C Porphyromonas gingivalis
1.3.1.10 0.0478
-
 crotonyl-[acyl-carrier protein] pH 7.5, 25°C Porphyromonas gingivalis
1.3.1.39 0.017
-
 NADPH pH 7.5, 25°C Porphyromonas gingivalis
1.3.1.39 0.0478
-
 crotonyl-CoA pH 7.5, 25°C Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.10 33090
-
 x * 33090, calculated Porphyromonas gingivalis
1.3.1.39 33090
-
 x * 33090, calculated Porphyromonas gingivalis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.3.1.10 dimethyl sulfoxide up to 10%, no loss of activity Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.10 Porphyromonas gingivalis Q7MAW0
-
-
1.3.1.10 Porphyromonas gingivalis W83 Q7MAW0
-
-
1.3.1.39 Porphyromonas gingivalis Q7MAW0
-
-
1.3.1.39 Porphyromonas gingivalis W83 Q7MAW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.10 recombinant protein Porphyromonas gingivalis

Storage Stability

EC Number Storage Stability Organism
1.3.1.10 -20°C, 30% glycerol, no significant loss of activity for at least 1 month Porphyromonas gingivalis
1.3.1.39 -20°C, 5 mg/ml concentration, 30% glycerol stock, stable for at least 1 month Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.10 crotonyl-[acyl-carrier protein] + NADPH + H+
-
 Porphyromonas gingivalis butyryl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.10 crotonyl-[acyl-carrier protein] + NADPH + H+
-
 Porphyromonas gingivalis W83 butyryl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.10 additional information enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate Porphyromonas gingivalis ?
-
 ?
1.3.1.10 additional information enzyme possesses intrinsic NADPH oxidase activity in the absence of the crotonyl-CoA substrate Porphyromonas gingivalis W83 ?
-
 ?
1.3.1.39 crotonyl-CoA + NADPH + H+
-
 Porphyromonas gingivalis butyryl-CoA + NADP+
-
 ?
1.3.1.39 crotonyl-CoA + NADPH + H+
-
 Porphyromonas gingivalis W83 butyryl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.10 ? x * 33090, calculated Porphyromonas gingivalis
1.3.1.39 ? x * 33090, calculated Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.10 enoyl-ACP-reductase II
-
 Porphyromonas gingivalis
1.3.1.10 FabK
-
 Porphyromonas gingivalis
1.3.1.39 FabK
-
 Porphyromonas gingivalis
1.3.1.39 PG1416 locus name Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.10 7.5
-
-
 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.10 FMN
-
 Porphyromonas gingivalis
1.3.1.10 NADH poor cofactor Porphyromonas gingivalis
1.3.1.10 NADPH inhibitory at high concentration Porphyromonas gingivalis
1.3.1.39 NADPH
-
 Porphyromonas gingivalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.10 0.109
-
 NADPH pH 7.5, 25°C Porphyromonas gingivalis