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Literature summary extracted from

  • Ku, H.; Park, S.; Yang, I.; Kim, S.
    Expression and functional characterization of prephenate dehydrogenase from Streptococcus mutans (2010), Process Biochem., 45, 607-612.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.12 expression in Escherichia coli Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.12 L-tyrosine 0.1 mM, less than 20% residual activity Streptococcus mutans
1.3.1.12 additional information not inhibitory: NaCl up to 10 mM, EDTA and dithiothreitol up to 1 mM Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.12 0.75
-
 prephenate pH 6.8, 37°C Streptococcus mutans
1.3.1.12 3.7
-
 NAD+ pH 6.8, 37°C Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.12 41000
-
 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans
1.3.1.12 41800
-
 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans
1.3.1.12 66000
-
 gel filtration Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 Streptococcus mutans Q8DUW0
-
-
1.3.1.12 Streptococcus mutans ATCC 700610 Q8DUW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 recombinant protein Streptococcus mutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.12 12.6
-
 pH 6.8, 37°C Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
1.3.1.12 4°C, -20°C, or -80°C, 50 mM Tris, 25% glycerol, no significant differences in activity, even after one month of storage Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 Streptococcus mutans 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?
1.3.1.12 prephenate + NAD+
-
 Streptococcus mutans ATCC 700610 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.12 dimer 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.12 37
-
-
 Streptococcus mutans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.12 7.4
-
 NAD+ pH 6.8, 37°C Streptococcus mutans
1.3.1.12 10.5
-
 prephenate pH 6.8, 37°C Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.12 6.8
-
-
 Streptococcus mutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.12 8
-
 50% of maximum activity Streptococcus mutans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.12 2
-
 NAD+ pH 6.8, 37°C Streptococcus mutans
1.3.1.12 13.9
-
 prephenate pH 6.8, 37°C Streptococcus mutans