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Literature summary extracted from

  • Moore, S.; Lawrence, A.; Biedendieck, R.; Deery, E.; Frank, S.; Howard, M.; Rigby, S.; Warren, M.
    Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12) (2013), Proc. Natl. Acad. Sci. USA, 110, 14906-14911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.106 homologously overproduced enzyme Priestia megaterium
2.1.1.195 expressed in Bacillus megaterium DSM319 cells Priestia megaterium
5.4.99.60 homologously overproduced enzyme Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.271 additional information by combining the enzymes CbiH60, CbiF, and CbiG with the cofactors SAM and dithionite, and the substrate cobalt-factor III in an enzyme-mixture incubation over 80% transformation of cobalt-factor III into cobalt-precorrin-5B can be achieved Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.106 cobalt-precorrin-6A + NADH + H+ Priestia megaterium the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis cobalt-precorrin-6B + NAD+
-
 ?
1.3.1.106 cobalt-precorrin-6A + NADH + H+ Priestia megaterium DSM 509 the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis cobalt-precorrin-6B + NAD+
-
 ?
2.1.1.195 cobalt-precorrin-5B + S-adenosyl-L-methionine Priestia megaterium
-
 cobalt-precorrin-6A + S-adenosyl-L-homocysteine
-
 ir
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4 Priestia megaterium cobalt-precorrin-4 has is a pathway intermediate of cobinamide biosynthesis S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4 Priestia megaterium DSM 509 cobalt-precorrin-4 has is a pathway intermediate of cobinamide biosynthesis S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.289 cobalt-precorrin-7 + S-adenosyl-L-methionine Priestia megaterium
-
 cobalt-precorrin-8 + S-adenosyl-L-homocysteine
-
 ?
2.1.1.289 cobalt-precorrin-7 + S-adenosyl-L-methionine Priestia megaterium DSM 509
-
 cobalt-precorrin-8 + S-adenosyl-L-homocysteine
-
 ?
5.4.99.60 cobalt-precorrin-8 Priestia megaterium the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis cobyrinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.106 Priestia megaterium
-
-
-
1.3.1.106 Priestia megaterium DSM 509
-
-
-
2.1.1.195 Priestia megaterium Q87693
-
-
2.1.1.271 Priestia megaterium
-
 gene cbiF
-
2.1.1.271 Priestia megaterium DSM 509
-
 gene cbiF
-
2.1.1.289 Priestia megaterium
-
-
-
5.4.99.60 Priestia megaterium
-
-
-
5.4.99.60 Priestia megaterium DSM 509
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.106 cobalt-precorrin-6A + NADH + H+ no activity with NADPH Priestia megaterium cobalt-precorrin-6B + NAD+
-
 ?
1.3.1.106 cobalt-precorrin-6A + NADH + H+ the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis Priestia megaterium cobalt-precorrin-6B + NAD+
-
 ?
1.3.1.106 cobalt-precorrin-6A + NADH + H+ no activity with NADPH Priestia megaterium DSM 509 cobalt-precorrin-6B + NAD+
-
 ?
1.3.1.106 cobalt-precorrin-6A + NADH + H+ the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis Priestia megaterium DSM 509 cobalt-precorrin-6B + NAD+
-
 ?
2.1.1.195 cobalt-precorrin-5B + S-adenosyl-L-methionine
-
 Priestia megaterium cobalt-precorrin-6A + S-adenosyl-L-homocysteine
-
 ir
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4 cobalt-precorrin-4 has is a pathway intermediate of cobinamide biosynthesis Priestia megaterium S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4
-
 Priestia megaterium DSM 509 S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4 cobalt-precorrin-4 has is a pathway intermediate of cobinamide biosynthesis Priestia megaterium DSM 509 S-adenosyl-L-homocysteine + cobalt-precorrin-5A
-
 ?
2.1.1.289 cobalt-precorrin-7 + S-adenosyl-L-methionine
-
 Priestia megaterium cobalt-precorrin-8 + S-adenosyl-L-homocysteine
-
 ?
2.1.1.289 cobalt-precorrin-7 + S-adenosyl-L-methionine
-
 Priestia megaterium DSM 509 cobalt-precorrin-8 + S-adenosyl-L-homocysteine
-
 ?
5.4.99.60 cobalt-precorrin-8
-
 Priestia megaterium cobyrinate
-
 ?
5.4.99.60 cobalt-precorrin-8 the enzyme catalyses a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis Priestia megaterium cobyrinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.106 CbiJ
-
 Priestia megaterium
2.1.1.195 CbiD
-
 Priestia megaterium
2.1.1.271 CbiF
-
 Priestia megaterium
2.1.1.289 CbiET
-
 Priestia megaterium
5.4.99.60 CbiC
-
 Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.106 NADH no activity with NADPH Priestia megaterium
2.1.1.271 S-adenosyl-L-methionine
-
 Priestia megaterium

General Information

EC Number General Information Comment Organism
2.1.1.271 metabolism the enzyme is involved in the cobinamide biosynthesis Priestia megaterium