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Literature summary extracted from

  • Singhal, A.; Choudhary, G.; Thakur, I.S.
    Characterization of laccase activity produced by Cryptococcus albidus (2012), Prep. Biochem. Biotechnol., 42, 113-124.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 1,4-diethylenedioxide complete inhibition at 50% or above Naganishia albida
1.10.3.2 2-mercaptoethanol complete inhibition of laccase activity at 0.1% concentration Naganishia albida
1.10.3.2 acetone complete inhibition at 50% or above Naganishia albida
1.10.3.2 acetonitrile complete inhibition at 50% or above Naganishia albida
1.10.3.2 methanol complete inhibition at 50% or above Naganishia albida
1.10.3.2 Sodium azide complete inhibition of laccase activity at 0.1% concentration Naganishia albida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information Michaelis-Menten kinetics Naganishia albida
1.10.3.2 0.8158
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 2.5, 25°C Naganishia albida

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 1,4-dioxan complete inhibition at 50% or above Naganishia albida
1.10.3.2 Acetone complete inhibition at 50% or above Naganishia albida
1.10.3.2 acetonitrile complete inhibition at 50% or above Naganishia albida
1.10.3.2 Methanol complete inhibition at 50% or above Naganishia albida

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Naganishia albida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Naganishia albida ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 20 30 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Naganishia albida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 25 65 half-life of 81 min at 25°C, 77 min at 35°C, 64 min at 45°C, 36 min at 55°C, and 21 min at 65°C Naganishia albida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.5
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Naganishia albida