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Literature summary extracted from

  • Märtens, B.; Amman, F.; Manoharadas, S.; Zeichen, L.; Orell, A.; Albers, S.V.; Hofacker, I.; Bläsi, U.
    Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2 (2013), PLoS One, 8, e76569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.B1 expression in Escherichia col Sulfolobus acidocaldarius

Protein Variants

EC Number Protein Variants Comment Organism
3.1.13.B1 additional information to address the biological significance of Saci-aCPSF2, a deletion mutant is constructed, and the influence of SaciaCPSF2 on the transcriptome profile is assessed employing high throughput RNA sequencing Sulfolobus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.B1 Mg2+ activity is dependent on Mg2+ Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.B1 RNA + H2O Sulfolobus acidocaldarius the enzyme plays a major role in mRNA turnover 3'-phosphomononucleotides
-
 ?
3.1.13.B1 RNA + H2O Sulfolobus acidocaldarius DSM 639 the enzyme plays a major role in mRNA turnover 3'-phosphomononucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.B1 Sulfolobus acidocaldarius Q4J6E1
-
-
3.1.13.B1 Sulfolobus acidocaldarius DSM 639 Q4J6E1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.B1
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.B1 5'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius ATP + 41 AMP
-
 ?
3.1.13.B1 5'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius DSM 639 ATP + 41 AMP
-
 ?
3.1.13.B1 RNA + H2O the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius 3'-phosphomononucleotides
-
 ?
3.1.13.B1 RNA + H2O the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius DSM 639 3'-phosphomononucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.13.B1 Saci-aCPSF2
-
 Sulfolobus acidocaldarius
3.1.13.B1 Saci2362 locus name Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.13.B1 65
-
 assay at Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.13.B1 7.5
-
 assay at Sulfolobus acidocaldarius

General Information

EC Number General Information Comment Organism
3.1.13.B1 physiological function the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius