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Literature summary extracted from

  • Krog, A.; Heggeset, T.M.; Mueller, J.E.; Kupper, C.E.; Schneider, O.; Vorholt, J.A.; Ellingsen, T.E.; Brautaset, T.
    Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties (2013), PLoS ONE, 8, e59188.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.244 activator protein ACT all three isozymes are activated by ACT, which is encoded by gene act, overview Bacillus methanolicus
1.1.1.244 NudF protein can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro Bacillus methanolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.244 three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566 Bacillus methanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.244 1
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh3 Bacillus methanolicus
1.1.1.244 1.1
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh1 Bacillus methanolicus
1.1.1.244 3
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh1 Bacillus methanolicus
1.1.1.244 4.5
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh2 Bacillus methanolicus
1.1.1.244 7
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh2 Bacillus methanolicus
1.1.1.244 7.1
-
 formaldehyde pH 6.7, 45°C, isozyme Mdh3 Bacillus methanolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.244 methanol + NAD+ Bacillus methanolicus
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Bacillus methanolicus PB1
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Bacillus methanolicus MGA3
-
 formaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.244 Bacillus methanolicus
-
 ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
1.1.1.244 Bacillus methanolicus
-
 NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
1.1.1.244 Bacillus methanolicus MGA3
-
 ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
1.1.1.244 Bacillus methanolicus PB1
-
 NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.244 1,3-propandiol + NAD+ very low activity Bacillus methanolicus ? + NADH + H+
-
 ?
1.1.1.244 1,3-propandiol + NAD+ low activity Bacillus methanolicus ? + NADH + H+
-
 ?
1.1.1.244 butanol + NAD+
-
 Bacillus methanolicus butanal + NADH + H+
-
 ?
1.1.1.244 ethanol + NAD+
-
 Bacillus methanolicus acetaldehyde + NADH + H+
-
 r
1.1.1.244 formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus methanol + NAD+
-
 r
1.1.1.244 formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 methanol + NAD+
-
 r
1.1.1.244 formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 methanol + NAD+
-
 r
1.1.1.244 isopropanol + NAD+ low activity Bacillus methanolicus isopropanal + NADH + H+
-
 ?
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
 r
1.1.1.244 additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus ?
-
 ?
1.1.1.244 additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus PB1 ?
-
 ?
1.1.1.244 additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus MGA3 ?
-
 ?
1.1.1.244 propanol + NAD+
-
 Bacillus methanolicus propanal + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.244 MDH
-
 Bacillus methanolicus
1.1.1.244 NAD+ dependent methanol dehydrogenase
-
 Bacillus methanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.244 45
-
 activity optimum at about 45°C for all isozymes Bacillus methanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.244 45
-
 20 min, the isozymes are all stable up to Bacillus methanolicus
1.1.1.244 45
-
 20 min, the isozymes are stable up to, except for isozyme Mdh1, which shows about 35-40% loss of activity Bacillus methanolicus
1.1.1.244 60
-
 20 min, isozyme Mdh1 is inactivated, isozyme Mdh2 loses about 80% of activity, while isozyme Mdh3 retains over 80% of its activity Bacillus methanolicus
1.1.1.244 60
-
 20 min, isozyme Mdh1 loses 90% activity, isozyme Mdh2 loses 80% of activity, while isozyme Mdh3 retains 60% of its activity Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.244 6.7
-
 formaldehyde reduction assay at Bacillus methanolicus
1.1.1.244 9.5
-
 alcohol oxidation reaction assay at Bacillus methanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.244 NAD+ dependent on Bacillus methanolicus
1.1.1.244 NADH
-
 Bacillus methanolicus

General Information

EC Number General Information Comment Organism
1.1.1.244 evolution strain MGA3 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes Bacillus methanolicus
1.1.1.244 evolution strain PB1 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes Bacillus methanolicus
1.1.1.244 physiological function biological significance of Mdh for methanol oxidation during methylotrophic growth and biological role of the enzyme as part of a formaldehyde detoxification system in the methanol consuming cells Bacillus methanolicus