EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.1 | recombinant expression of C-terminally His6-tagged or N-termminally His8-tagged enzyme in Thermus thermophilus under the control of a constitutive promoter | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | 3-nitropropionic acid | - |
Thermus thermophilus | |
1.3.5.1 | malonate | - |
Thermus thermophilus | |
1.3.5.1 | nonyl-4-hydroxyquinoline-N-oxide | the semiquinone analog and inhibitor of quinone reactions in complex II shows no influence on the redox behavior of the heme b moieties | Thermus thermophilus | |
1.3.5.1 | oxaloacetate | - |
Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.1 | additional information | - |
additional information | interprotomer temperature-dependent positive cooperativity in the trimeric complex. Only the trimer, not the monomer, exhibits positive cooperativity at high temperatures | Thermus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.5.1 | membrane | - |
Thermus thermophilus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | Fe2+ | the enzyme contains a [3Fe-4S] center | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.5.1 | 119800 | - |
monomeric recombinant C-terminally His6-tagged enzyme, gel filtration | Thermus thermophilus |
1.3.5.1 | 120000 | - |
1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
1.3.5.1 | 120000 | - |
3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
1.3.5.1 | 120000 | - |
4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
1.3.5.1 | 500000 | - |
wild-type enzyme, consisting of 360 kDa from protein and an unknown contribution of detergent and lipid, native PAGE | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.1 | succinate + menaquinone | Thermus thermophilus | - |
fumarate + menaquinol | - |
? | |
1.3.5.1 | succinate + menaquinone | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
fumarate + menaquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.1 | Thermus thermophilus | - |
- |
- |
1.3.5.1 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.5.1 | recombinant His6- or His8-tagged enzyme from Thermus thermophilus by nickel affinity chromatography and gel filtration, untagged enzyme by anion exchange chromatography and gel filtration | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.1 | succinate + menaquinone | - |
Thermus thermophilus | fumarate + menaquinol | - |
? | |
1.3.5.1 | succinate + menaquinone | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | fumarate + menaquinol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.5.1 | monomer | 1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
1.3.5.1 | tetramer | 4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
1.3.5.1 | trimer | 3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.1 | SQR | - |
Thermus thermophilus |
1.3.5.1 | succinate:quinone reductase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.5.1 | 30 | - |
assay at | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.5.1 | 7.6 | - |
assay at | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | flavin | covalently-bound flavin cofactor | Thermus thermophilus | |
1.3.5.1 | heme | the enzyme contains two heme b cofactors, a di-heme | Thermus thermophilus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.5.1 | physiological function | succinate:quinone reductase serves as the respiratory complex II | Thermus thermophilus |