Silencing of flavanone-3-hydroxylase in apple (Malus x domestica Borkh.) leads to accumulation of flavanones, but not to reduced fire blight susceptibility
Flachowsky, H.; Halbwirth, H.; Treutter, D.; Richter, K.; Hanke, M.V.; Szankowski, I.; Gosch, C.; Stich, K.; Fischer, T.C.; Plant Physiol. Biochem. 51, 18-25 (2012) 
Data extracted from this reference:
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene fht, transcription profiling and quantitative real-time PCR expression analysis
Malus domestica
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
F3H silencing by fht antisense transgenic apple clones construction, diverse transgenic lines, phenotypes, overview
Malus domestica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.9
prohexadione-Ca
an enzyme inhibitor for 2-oxoglutarate dependent dioxygenases, used as a growth retardant and for control of secondary fire blight, Erwinia amylovora, of apple leaves
Malus domestica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required
Malus domestica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a flavanone + 2-oxoglutarate + O2
Malus domestica
-
a dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodyctiol + 2-oxoglutarate + O2
Malus domestica
-
dihydroquercetin + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Malus domestica
-
cv. Pinova, gene fht
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a flavanone + 2-oxoglutarate + O2
-
726200
Malus domestica
a dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodyctiol + 2-oxoglutarate + O2
-
726200
Malus domestica
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
726200
Malus domestica
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H
-
Malus domestica
1.14.11.9
FHT
-
Malus domestica
1.14.11.9
flavanone-3-hydroxylase
-
Malus domestica
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.9
30
-
assay at
Malus domestica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.9
7.25
-
assay at
Malus domestica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene fht, transcription profiling and quantitative real-time PCR expression analysis
Malus domestica
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
F3H silencing by fht antisense transgenic apple clones construction, diverse transgenic lines, phenotypes, overview
Malus domestica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.9
prohexadione-Ca
an enzyme inhibitor for 2-oxoglutarate dependent dioxygenases, used as a growth retardant and for control of secondary fire blight, Erwinia amylovora, of apple leaves
Malus domestica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a flavanone + 2-oxoglutarate + O2
Malus domestica
-
a dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodyctiol + 2-oxoglutarate + O2
Malus domestica
-
dihydroquercetin + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a flavanone + 2-oxoglutarate + O2
-
726200
Malus domestica
a dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodyctiol + 2-oxoglutarate + O2
-
726200
Malus domestica
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
726200
Malus domestica
dihydrokaempferol + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.9
30
-
assay at
Malus domestica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.11.9
7.25
-
assay at
Malus domestica
Expression
EC Number
Organism
Commentary
Expression
1.14.11.9
Malus domestica
downregulation of flavanone 4-reductase leads to upregulation of other flavanoid pathway genes, inclunding the flavanone 3-hydroxylase, feedback regulation of flavonoid gene expression, overview
up
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the 2-oxoglutarate-dependant dioxygenases
Malus domestica
1.14.11.9
malfunction
silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-deoxyflavonoid luteoforol is formed from accumulating flavanones, acting as an antimicrobial compound against the fire blight pathogen Erwinia amylovora. Inducible resistance to fire blight by prohexadione-Ca is not observed with the antisense flavanone-3-hydroxylase apple plants
Malus domestica
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the 2-oxoglutarate-dependant dioxygenases
Malus domestica
1.14.11.9
malfunction
silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-deoxyflavonoid luteoforol is formed from accumulating flavanones, acting as an antimicrobial compound against the fire blight pathogen Erwinia amylovora. Inducible resistance to fire blight by prohexadione-Ca is not observed with the antisense flavanone-3-hydroxylase apple plants
Malus domestica
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.9
Malus domestica
downregulation of flavanone 4-reductase leads to upregulation of other flavanoid pathway genes, inclunding the flavanone 3-hydroxylase, feedback regulation of flavonoid gene expression, overview
up