Any feedback?
Literature summary extracted from
- Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng (2012), Plant Cell Physiol., 53, 1535-1545.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.121 | expressed in Saccharomyces cerevisiae WAT21 cells | Panax ginseng |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.121 | 55300 | - |
x * 55300, calculated from amino acid sequence | Panax ginseng |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.120 | dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | hydroxylation of dammarenediol-II at the C-12 position | protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.121 | protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | - |
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.120 | Panax ginseng | - |
cultivar C.A. Meyer | - |
| 1.14.14.121 | Panax ginseng | I7C6E8 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.120 | leaf | - |
Panax ginseng | - |
| 1.14.14.120 | plant | - |
Panax ginseng | - |
| 1.14.14.120 | root | - |
Panax ginseng | - |
| 1.14.14.121 | flower bud | - |
Panax ginseng | - |
| 1.14.14.121 | leaf | - |
Panax ginseng | - |
| 1.14.14.121 | petiole | - |
Panax ginseng | - |
| 1.14.14.121 | rhizome | highest expression | Panax ginseng | - |
| 1.14.14.121 | root | - |
Panax ginseng | - |
| 1.14.14.121 | sympodium | - |
Panax ginseng | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.120 | dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation of dammarenediol-II at the C-12 position | Panax ginseng | protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.121 | protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Panax ginseng | protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.121 | ? | x * 55300, calculated from amino acid sequence | Panax ginseng |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.120 | CYP716A47 | - |
Panax ginseng |
| 1.14.14.120 | protopanaxadiol synthase | - |
Panax ginseng |
| 1.14.14.121 | CYP716A53v2 | - |
Panax ginseng |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.14.120 | Panax ginseng | methyl jasmonate treatment results in accumulation of mRNA in adventitious roots | up |
| 1.14.14.121 | Panax ginseng | the enzyme is not affected by methyl jasmonate treatment | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.121 | metabolism | protopanaxadiol 6-hydroxylase produces protopanaxatriol from protopanaxadiol, which is an important step in the formation of dammarane-type triterpene aglycones in ginseng saponin biosynthesis | Panax ginseng |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
