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Literature summary extracted from

  • Gest, N.; Garchery, C.; Gautier, H.; Jimenez, A.; Stevens, R.
    Light-dependent regulation of ascorbate in tomato by a monodehydroascorbate reductase localized in peroxisomes and the cytosol (2013), Plant Biotechnol. J., 11, 344-354.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 gene MDHAR3, functional overexpression in transgenic Solanum lycopersicum plant leaves, quantitative reverse-transcriptase PCR expression analysis Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.4 additional information transgenic lines overexpressing MDHAR show a decrease in ascorbate levels in leaves, whereas lines where MDHAR is silenced show an increase in these levels in both fruits and leave. In all three lines where MDHAR3 is silenced (MDS lines), a significant decrease in overall MDHAR activity is observed in leavess. Transcript levels of MDHAR1 (SGN-U584073) and MDHAR2 (SGN-U583672) remain unaffected by modification of MDHAR3 Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 cytosol
-
 Solanum lycopersicum 5829
-
1.6.5.4 additional information MDHAR isoforms in both the cytosol and peroxisome Solanum lycopersicum
-
-
1.6.5.4 peroxisome isozyme MDHAR3 Solanum lycopersicum 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.4 NADH + H+ + 2 monodehydroascorbate Solanum lycopersicum
-
 NAD+ + 2 ascorbate
-
 ?
1.6.5.4 NADH + H+ + 2 monodehydroascorbate Solanum lycopersicum West Virginia 106
-
 NAD+ + 2 ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Solanum lycopersicum
-
-
-
1.6.5.4 Solanum lycopersicum West Virginia 106
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 fruit
-
 Solanum lycopersicum
-
1.6.5.4 leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NADH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum NAD+ + 2 ascorbate
-
 ?
1.6.5.4 NADH + H+ + 2 monodehydroascorbate
-
 Solanum lycopersicum West Virginia 106 NAD+ + 2 ascorbate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDHAR
-
 Solanum lycopersicum
1.6.5.4 monodehydroascorbate reductase
-
 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
1.6.5.4 evolution in plants, MDHAR belongs to a multigene family Solanum lycopersicum
1.6.5.4 malfunction transgenic lines overexpressing MDHAR show a decrease in ascorbate levels in leaves, whereas lines where MDHAR is silenced show an increase in these levels in both fruits and leaves Solanum lycopersicum
1.6.5.4 metabolism the enzyme is involved in the ascorbate recycling pathway, overview Solanum lycopersicum
1.6.5.4 physiological function the enzyme negatively regulates ascorbate levels in Solanum lycopersicum, isozyme MDHAR3 activity has a negative impact on ascorbate levels in both leaves and fruits Solanum lycopersicum