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Literature summary extracted from

  • Sartori, A.A.; Lingaraju, G.M.; Hunziker, P.; Winkler, F.K.; Jiricny, J.
    Pa-AGOG, the founding member of a new family of archaeal 8-oxoguanine DNA-glycosylases (2004), Nucleic Acids Res., 32, 6531-6539.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.B5 expression in Escherichia coli Pyrobaculum aerophilum
4.2.99.18 expression in Escherichia coli Pyrobaculum aerophilum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.B5 D166N mutant enzyme shows wild-type activity Pyrobaculum aerophilum
3.2.2.B5 D172N inactive mutant enzyme Pyrobaculum aerophilum
3.2.2.B5 K140Q inactive mutant enzyme Pyrobaculum aerophilum
3.2.2.B5 K147Q activity of mutant enzyme is severely attenuated Pyrobaculum aerophilum
4.2.99.18 D166N mutant enzyme shows wild-type activity Pyrobaculum aerophilum
4.2.99.18 D172N inactive mutant enzyme Pyrobaculum aerophilum
4.2.99.18 K140Q inactive mutant enzyme Pyrobaculum aerophilum
4.2.99.18 K147Q activity of mutant enzyme is severely attenuated Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.B5 29467
-
 x * 29467, calculated from sequence Pyrobaculum aerophilum
4.2.99.18 29467
-
 x * 29467, calculated from sequence Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O Pyrobaculum aerophilum the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O Pyrobaculum aerophilum DSM 7523 the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates DNA with an abasic site + 8-oxoguanine
-
 ?
4.2.99.18 DNA with an abasic site Pyrobaculum aerophilum the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates ?
-
 ?
4.2.99.18 DNA with an abasic site Pyrobaculum aerophilum DSM 7523 the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.B5 Pyrobaculum aerophilum Q8ZVK6
-
-
3.2.2.B5 Pyrobaculum aerophilum DSM 7523 Q8ZVK6
-
-
4.2.99.18 Pyrobaculum aerophilum Q8ZVK6
-
-
4.2.99.18 Pyrobaculum aerophilum DSM 7523 Q8ZVK6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.B5
-
 Pyrobaculum aerophilum
4.2.99.18
-
 Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O at 60°C, the enzyme is able to process more than 90% of the 8-oxoguanine/guanine and 8-oxoguanine/cytosine substrates in 15 min, at a substrate to enzyme ratio of 10:1 Pyrobaculum aerophilum DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum DSM 7523 DNA with an abasic site + 8-oxoguanine
-
 ?
3.2.2.B5 DNA containing 8-oxoguanine + H2O at 60°C, the enzyme is able to process more than 90% of the 8-oxoguanine/guanine and 8-oxoguanine/cytosine substrates in 15 min, at a substrate to enzyme ratio of 10:1 Pyrobaculum aerophilum DSM 7523 DNA with an abasic site + 8-oxoguanine
-
 ?
4.2.99.18 DNA with an abasic site the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum ?
-
 ?
4.2.99.18 DNA with an abasic site the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point Pyrobaculum aerophilum ?
-
 ?
4.2.99.18 DNA with an abasic site the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates Pyrobaculum aerophilum DSM 7523 ?
-
 ?
4.2.99.18 DNA with an abasic site the substrate specificity for 8-oxoguanine/guanine sites is higher than that for 8-oxoguanine/cytosine sites. apurinic-lyase activity of this enzyme cleaved only 20% of these apurinic-sites at the same time point Pyrobaculum aerophilum DSM 7523 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.B5 ? x * 29467, calculated from sequence Pyrobaculum aerophilum
4.2.99.18 ? x * 29467, calculated from sequence Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
3.2.2.B5 GO-glycosylase
-
 Pyrobaculum aerophilum
3.2.2.B5 Pa-AGOG bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum
3.2.2.B5 PAE2237 locus name Pyrobaculum aerophilum
4.2.99.18 Pa-AGOG bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum
4.2.99.18 PAE2237 locus name Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.B5 80
-
 15 min, no notable effect on catalytic activity Pyrobaculum aerophilum
4.2.99.18 80
-
 15 min, no notable effect on catalytic activity Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.B5 9.2
-
 calculated from sequence Pyrobaculum aerophilum
4.2.99.18 9.2
-
 calculated from sequence Pyrobaculum aerophilum

General Information

EC Number General Information Comment Organism
3.2.2.B5 physiological function the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates. Bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum
4.2.99.18 physiological function the enzyme is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates. Bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18) Pyrobaculum aerophilum