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Literature summary extracted from

  • Bao, S.; Teng, Z.; Ding, S.
    Heterologous expression and characterization of a novel laccase isoenzyme with dyes decolorization potential from Coprinus comatus (2013), Mol. Biol. Rep., 40, 1927-1936.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 gene lac2, DNA and amino acid sequence determination and analysis Coprinus comatus
1.10.3.2 genes lac1 and lac2, DNA and amino acid sequence determination and analysis, expression of Lac1 in Pichia pastoris Coprinus comatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 DTT complete inhibition at 5 mM Coprinus comatus
1.10.3.2 NaN3 complete inhibition at 5 mM Coprinus comatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.014
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 0.034
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 4.317
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 7.611
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Fe2+ slightly activating Coprinus comatus
1.10.3.2 K+ slightly activating Coprinus comatus
1.10.3.2 NH4+ slightly activating Coprinus comatus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Coprinus comatus H9C4A2 lac2; gene lac2
-
1.10.3.2 Coprinus comatus H9C4A3 lac1; genes lac1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant enzyme Lac1 from Pichia pastoris by ultrafiltration, anion exchange chromatography, ultrafiltration, and gel filtration Coprinus comatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 2,6-dimethylphenol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 4 2,6-dimethylphenol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 4 guaiacol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 4 syringaldazine + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 additional information the enzyme shows dye decolorization potential, overview Coprinus comatus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Coprinus comatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 LAC1
-
 Coprinus comatus
1.10.3.2 Lac2
-
 Coprinus comatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
 with substrates syringaldazine, recombinant Lac1 Coprinus comatus
1.10.3.2 55
-
 with substrates guaiacol, recombinant Lac1 Coprinus comatus
1.10.3.2 65
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 Coprinus comatus
1.10.3.2 70
-
 with substrate 2,6-dimethylphenol, recombinant Lac1 Coprinus comatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 1.15
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 7.67
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 15.93
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1 Coprinus comatus
1.10.3.2 5.5
-
 with substrate 2,6-dimethylphenol, recombinant Lac1 Coprinus comatus
1.10.3.2 6
-
 with substrates guaiacol and syringaldazine, recombinant Lac1 Coprinus comatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.15
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 1.78
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 42.55
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus
1.10.3.2 465.8
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, recombinant Lac1 Coprinus comatus