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Literature summary extracted from

  • Meza-Cervantez, P.; Gonzalez-Robles, A.; Cardenas-Guerra, R.E.; Ortega-Lopez, J.; Saavedra, E.; Pineda, E.; Arroyo, R.
    Pyruvate:ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells (2011), Microbiology, 157, 3469-3482.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.1 expressed in Escherichia coli BL21(DE3) cells Trichomonas vaginalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.1 cytosol about 1% of activity detected in cytosol Trichomonas vaginalis 5829
-
1.2.7.1 hydrogenosome 80% of activity detected in hydrogenosome Trichomonas vaginalis 42566
-
1.2.7.1 membrane about 4% of activity detected in membrane Trichomonas vaginalis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 120000
-
 x * 120000, pyruvate : ferredoxin oxidoreductase A, SDS-PAGE Trichomonas vaginalis

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Trichomonas vaginalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 Ni-NTA column chromatography Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + oxidized nitro blue tetrazolium
-
 Trichomonas vaginalis acetyl-CoA + CO2 + reduced nitro blue tetrazolium + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 ? x * 120000, pyruvate : ferredoxin oxidoreductase A, SDS-PAGE Trichomonas vaginalis

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 AP120
-
 Trichomonas vaginalis
1.2.7.1 PFO A
-
 Trichomonas vaginalis
1.2.7.1 pyruvate:ferredoxin oxidoreductase A
-
 Trichomonas vaginalis

General Information

EC Number General Information Comment Organism
1.2.7.1 physiological function the enzyme is a surface-associated cell-binding protein that lacks enzyme activity and is involved in cytoadherence to host cells Trichomonas vaginalis