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Literature summary extracted from

  • Ramos, A.; Lombo, F.; Brana, A.F.; Rohr, J.; Mendez, C.; Salas, J.A.
    Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster (2008), Microbiology, 154, 781-788.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.305 expression in Streptomyces albus Streptomyces olivaceus
2.1.1.306 expression in Streptomyces albus Streptomyces olivaceus
2.1.1.307 expression in Streptomyces albus Streptomyces olivaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.305 41180
-
 x * 41180, calculated from sequence Streptomyces olivaceus
2.1.1.306 42836
-
 x * 42836, calculated from sequence Streptomyces olivaceus
2.1.1.307 29044
-
 x * 29044, calculated from sequence Streptomyces olivaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C Streptomyces olivaceus the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C Streptomyces olivaceus Tü2353 the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C Streptomyces olivaceus the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C Streptomyces olivaceus Tü2353 the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C Streptomyces olivaceus the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3,4-O-trimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C Streptomyces olivaceus Tü2353 the enzyme is involved in the biosynthesis of the polyketide elloramycin S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3,4-O-trimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.305 Streptomyces olivaceus Q9AJU2
-
-
2.1.1.305 Streptomyces olivaceus Tü2353 Q9AJU2
-
-
2.1.1.306 Streptomyces olivaceus Q9AJU1
-
-
2.1.1.306 Streptomyces olivaceus Tü2353 Q9AJU1
-
-
2.1.1.307 Streptomyces olivaceus Q9AJU0
-
-
2.1.1.307 Streptomyces olivaceus Tü2353 Q9AJU0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C using as biotransformation host strain EPM1 (expressing elmMI) about 24% of the precursor 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C is converted into 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C. No activity with substrates lacking the hydroxy group at C-2' (8-demethyl-8-alpha-L-oleandrosyl tetracenomycin C or 8-demethyl-8-alpha-L-olivosyl tetracenomycin C) Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.305 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl-tetracenomycin C using as biotransformation host strain EPM1 (expressing elmMI) about 24% of the precursor 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C is converted into 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C. No activity with substrates lacking the hydroxy group at C-2' (8-demethyl-8-alpha-L-oleandrosyl tetracenomycin C or 8-demethyl-8-alpha-L-olivosyl tetracenomycin C) Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 additional information no activity with 8-demethyl-8-alpha-L-oleandrosyl tetracenomycin C (which is already methylated at the 3'-hydroxy group) Streptomyces olivaceus ?
-
 ?
2.1.1.306 additional information no activity with 8-demethyl-8-alpha-L-oleandrosyl tetracenomycin C (which is already methylated at the 3'-hydroxy group) Streptomyces olivaceus Tü2353 ?
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-(2-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-olivosyl tetracenomycin C
-
 Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(3-O-methyl-alpha-L-olivosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-olivosyl tetracenomycin C
-
 Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(3-O-methyl-alpha-L-olivosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C
-
 Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(3-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.306 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C
-
 Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(3-O-methyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3,4-O-trimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)-tetracenomycin C the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(2,3,4-O-trimethyl-alpha-L-rhamnosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-oleandrosyl-tetracenomycin C no activity with 8-demethyl-8-alpha-L-olivosyl tetracenomycin C or 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C Streptomyces olivaceus S-adenosyl-L-homocysteine + 8-demethyl-8-(4-O-methyl-alpha-L-oleandrosyl)-tetracenomycin C
-
 ?
2.1.1.307 S-adenosyl-L-methionine + 8-demethyl-8-alpha-L-oleandrosyl-tetracenomycin C no activity with 8-demethyl-8-alpha-L-olivosyl tetracenomycin C or 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin C Streptomyces olivaceus Tü2353 S-adenosyl-L-homocysteine + 8-demethyl-8-(4-O-methyl-alpha-L-oleandrosyl)-tetracenomycin C
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.305 ? x * 41180, calculated from sequence Streptomyces olivaceus
2.1.1.306 ? x * 42836, calculated from sequence Streptomyces olivaceus
2.1.1.307 ? x * 29044, calculated from sequence Streptomyces olivaceus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.305 ElmMI
-
 Streptomyces olivaceus
2.1.1.306 ElmMII
-
 Streptomyces olivaceus
2.1.1.307 ElmMIII
-
 Streptomyces olivaceus

General Information

EC Number General Information Comment Organism
2.1.1.305 physiological function the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus
2.1.1.306 physiological function the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus
2.1.1.307 physiological function the enzyme is involved in the biosynthesis of the polyketide elloramycin Streptomyces olivaceus