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Literature summary extracted from

  • Harreither, W.; Nicholls, P.; Sygmund, C.; Gorton, L.; Ludwig, R.
    Investigation of the pH-dependent electron transfer mechanism of ascomycetous class II cellobiose dehydrogenases on electrodes (2012), Langmuir, 28, 6714-6723.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Neurospora crassa
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Thermothelomyces fergusii
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Stachybotrys bisbyi
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Amesia atrobrunnea
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Dichomera saubinetii
1.1.99.18 biotechnology class II cellobiose dehydrogenases are potential candidates for glucose biosensors and biofuel cell anodes Hypoxylon haematostroma

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.002
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.0024
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.003
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 0.0046
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 0.0049
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.005
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.0056
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.0057
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.0058
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.007
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.007
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.008
-
 cytochrome c at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.0087
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 0.0092
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.012
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.014
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 0.018
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.0259
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.028
-
 lactose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.032
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.048
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.05
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.0537
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.0698
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 2.4
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 7.1
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 7.8
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 13
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 21
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 24
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 31
-
 maltose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 170
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 230
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 260
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 770
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 1700
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Amesia atrobrunnea
-
-
-
1.1.99.18 Dichomera saubinetii
-
-
-
1.1.99.18 Hypoxylon haematostroma
-
-
-
1.1.99.18 Neurospora crassa
-
-
-
1.1.99.18 Stachybotrys bisbyi
-
-
-
1.1.99.18 Thermothelomyces fergusii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + cytochrome c
-
 Neurospora crassa cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Thermothelomyces fergusii cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Stachybotrys bisbyi cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Amesia atrobrunnea cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Dichomera saubinetii cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Hypoxylon haematostroma cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces fergusii D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Stachybotrys bisbyi D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Amesia atrobrunnea D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Dichomera saubinetii D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol
-
 Hypoxylon haematostroma D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Neurospora crassa ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Thermothelomyces fergusii ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Stachybotrys bisbyi ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Amesia atrobrunnea ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Dichomera saubinetii ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Hypoxylon haematostroma ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Neurospora crassa
1.1.99.18 Cdh
-
 Thermothelomyces fergusii
1.1.99.18 Cdh
-
 Stachybotrys bisbyi
1.1.99.18 Cdh
-
 Amesia atrobrunnea
1.1.99.18 Cdh
-
 Dichomera saubinetii
1.1.99.18 Cdh
-
 Hypoxylon haematostroma

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.2
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.3
-
 maltose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.5
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 1
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 1.2
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 1.6
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 1.9
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 2.1
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 2.3
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 3.3
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.4
-
 lactose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 3.6
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 3.9
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 4
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 4.9
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 5
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 7.1
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 7.7
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 8.2
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 9.2
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 9.4
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 10
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 10.9
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 11.8
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 13.5
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 16.2
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 16.2
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 17
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 18.1
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 21.3
-
 cytochrome c at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 21.4
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 22.6
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 24.8
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 25.2
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 28.7
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 53.8
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Neurospora crassa
1.1.99.18 FAD
-
 Thermothelomyces fergusii
1.1.99.18 FAD
-
 Stachybotrys bisbyi
1.1.99.18 FAD
-
 Amesia atrobrunnea
1.1.99.18 FAD
-
 Dichomera saubinetii
1.1.99.18 FAD
-
 Hypoxylon haematostroma
1.1.99.18 heme b
-
 Neurospora crassa
1.1.99.18 heme b
-
 Thermothelomyces fergusii
1.1.99.18 heme b
-
 Stachybotrys bisbyi
1.1.99.18 heme b
-
 Amesia atrobrunnea
1.1.99.18 heme b
-
 Dichomera saubinetii
1.1.99.18 heme b
-
 Hypoxylon haematostroma

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 0.007
-
 D-glucose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.009
-
 maltose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 0.01
-
 maltose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.011
-
 D-glucose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 0.014
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.021
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.048
-
 D-glucose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 0.067
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.09
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.128
-
 D-glucose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.208
-
 maltose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 71
-
 lactose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 71
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 120
-
 lactose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 190
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 220
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 320
-
 cellobiose at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 450
-
 cellobiose at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 450
-
 maltose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 540
-
 cytochrome c at pH 6.0 and 30°C Neurospora crassa
1.1.99.18 700
-
 cytochrome c at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 760
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 780
-
 lactose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 850
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 1000
-
 cytochrome c at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 1700
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Stachybotrys bisbyi
1.1.99.18 1800
-
 2,6-dichlorophenol indophenol at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 2400
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 2700
-
 cellobiose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 2900
-
 2,6-dichlorophenol indophenol at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 3100
-
 cytochrome c at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 3300
-
 lactose at pH 5.0 and 30°C Amesia atrobrunnea
1.1.99.18 3900
-
 2,6-dichlorophenol indophenol at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 4000
-
 cellobiose at pH 7.5 and 30°C Thermothelomyces fergusii
1.1.99.18 4400
-
 cytochrome c at pH 5.0 and 30°C Dichomera saubinetii