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Literature summary extracted from

  • Cho, H.J.; Cho, H.Y.; Kim, K.J.; Kim, M.H.; Kim, S.W.; Kang, B.S.
    Structural and functional analysis of bacterial flavin-containing monooxygenase reveals its ping-pong-type reaction mechanism (2011), J. Struct. Biol., 175, 39-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.8 expression in Escherichia coli Methylophaga aminisulfidivorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.8 crystal structures of enzyme and enzyme in complex with NADP+, and a mutant Y207S, which lacks indole oxygenation activity, with and without indole. The crystal structures reveal overlapping binding sites for NADP+ and indole, suggestive of a double-displacement reaction mechanism. NADPH induces conformational changes in two active site motifs. One of the motifs contains Arg229, which participates in interactions with the phosphate group of NADPH and appears be a determinant of the preferential binding to NADPH rather than NADH. The second motif contains Tyr207 Methylophaga aminisulfidivorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.8 Y207S mutant exhibits very little indoxyl producing activity but the NADPH oxidase activity of the mutant is higher than that of the wild-type enzyme Methylophaga aminisulfidivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.8 indole inhibits NADPH oxidase activity Methylophaga aminisulfidivorans
1.14.13.8 NADPH inhibits the binding of indole and decreases indoxyl production Methylophaga aminisulfidivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.8 0.005
-
 indole wild-type, pH 8.0, temperature not specified in the publication Methylophaga aminisulfidivorans
1.14.13.8 0.008
-
 indole mutant Y207S, pH 8.0, temperature not specified in the publication Methylophaga aminisulfidivorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Methylophaga aminisulfidivorans Q83XK4
-
-
1.14.13.8 Methylophaga aminisulfidivorans SK1 Q83XK4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 indole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans indoxyl + NADP+ + H2O
-
 ?
1.14.13.8 indole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans SK1 indoxyl + NADP+ + H2O
-
 ?