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Literature summary extracted from

  • Ahmad, A.; Low, E.; Shukor, S.
    Immobilization of phenylalanine dehydrogenase onto Eupergit CM for the synthesis of (S)-2-amino-4-phenylbutyric acid (2013), J. Mol. Catal. B, 88, 26-31.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.20 additional information covalent immobilization of phenylalanine dehydrogenase onto commercially available enzyme carrier Eupergit CM for the synthesis of unnatural amino acid (S)-2-amino-4-phenylbutyric acid, method optimization and evaluation, overview. The immobilized enzyme shows increased thermostability, activity, broadened pH optimum, and remarkable improvement in operability and storage stability compared to the native enzyme. Immobilized PheDH is successfully applied for the synthesis of (S)-2-amino-4-phenylbutyric acid, achieving enantiomeric excess of more than 99% and yield of more than 80%; comparable to synthesis using the free PheDH Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.20 L-phenylalanine + H2O + NAD+ Rhodococcus sp.
-
 phenylpyruvate + NH3 + NADH + H+
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+ Rhodococcus sp. 4
-
 phenylpyruvate + NH3 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Rhodococcus sp.
-
-
-
1.4.1.20 Rhodococcus sp. 4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-oxo-4-phenylbutanoic acid + NH3 + NADH + H+
-
 Rhodococcus sp. (S)-2-amino-4-phenylbutyric acid + H2O + NAD+
-
 r
1.4.1.20 2-oxo-4-phenylbutanoic acid + NH3 + NADH + H+
-
 Rhodococcus sp. 4 (S)-2-amino-4-phenylbutyric acid + H2O + NAD+
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Rhodococcus sp. phenylpyruvate + NH3 + NADH + H+
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Rhodococcus sp. 4 phenylpyruvate + NH3 + NADH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.20 50
-
 reductive amination, immobilized enzyme Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 7.5 9.5 reductive amination, immobilized enzyme Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Rhodococcus sp.
1.4.1.20 NADH
-
 Rhodococcus sp.