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Literature summary extracted from

  • Foster, A.; Barnes, N.; Speight, R.; Keane, M.
    Identification, functional expression and kinetic analysis of two primary amine oxidases from Rhodococcus opacus (2012), J. Mol. Catal. B, 74, 73-82.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 4-fluorobenzylamine
-
Rhodococcus opacus
1.4.3.21 aminoguanidine isoform AO1 shows 18% residual activity at 0.1 mM, isoform AO2 shows 17% residual activity at 0.1 mM Rhodococcus opacus
1.4.3.21 amylamine
-
Rhodococcus opacus
1.4.3.21 benzylamine
-
Rhodococcus opacus
1.4.3.21 Butylamine
-
Rhodococcus opacus
1.4.3.21 Cuprizone isoform AO1 shows 2% residual activity at 0.1 mM, isoform AO2 shows 1% residual activity at 0.1 mM Rhodococcus opacus
1.4.3.21 dopamine
-
Rhodococcus opacus
1.4.3.21 isoniazid isoform AO1 shows 14% residual activity at 0.1 mM, isoform AO2 shows 16% residual activity at 0.1 mM Rhodococcus opacus
1.4.3.21 additional information not inhibited by pargyline, clorgyline, and neocupine Rhodococcus opacus
1.4.3.21 octopamine
-
Rhodococcus opacus
1.4.3.21 Phenylethylamine
-
Rhodococcus opacus
1.4.3.21 Propylamine
-
Rhodococcus opacus
1.4.3.21 Semicarbazide isoform AO1 shows 1% residual activity at 0.1 mM, isoform AO2 shows 2% residual activity at 0.1 mM Rhodococcus opacus
1.4.3.21 tyramine
-
Rhodococcus opacus
1.4.3.21 vanillylamine
-
Rhodococcus opacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.02
-
vanillylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.02
-
Phenylethylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.03
-
octopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.04
-
benzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.05
-
tyramine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.1
-
dopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.11
-
amylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.13
-
4-fluorobenzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.16
-
amylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.18
-
methylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.28
-
histamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.32
-
Butylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.32
-
Butylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.49
-
Propylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.86
-
ethylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 2.05
-
ethanolamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Cu2+ contains copper Rhodococcus opacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.21 70000
-
2 * 70000, isoform AO1 or AO2, SDS-PAGE Rhodococcus opacus
1.4.3.21 71695
-
2 * 71695, isoform AO1, calculated from amino acid sequence Rhodococcus opacus
1.4.3.21 72051
-
2 * 72051, isoform AO2, calculated from amino acid sequence Rhodococcus opacus
1.4.3.21 140000
-
gel filtration Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Rhodococcus opacus
-
-
-
1.4.3.21 Rhodococcus opacus DSM 43250
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 ammonium sulfate precipitation, Hi-Prep 16 10 Q column chromatography, Mono Q column chromatography, and Superose 12 gel filtration Rhodococcus opacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.21 0.039
-
isoform AO1, crude extract, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.144
-
isoform AO2, crude extract, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 6.302
-
isoform AO1, after 161.5fold purification, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 13.14
-
isoform AO2, after 91.3fold purification, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 1-methyl-3-phenylpropylamine + H2O + O2
-
Rhodococcus opacus 4-phenylbutan-2-one + NH3 + H2O2
-
?
1.4.3.21 4-fluorobenzylamine + H2O + O2
-
Rhodococcus opacus 4-fluorobenzaldehyde + NH3 + H2O2
-
?
1.4.3.21 amphetamine + H2O + O2
-
Rhodococcus opacus 1-phenylpropan-2-one + NH3 + H2O2
-
?
1.4.3.21 amylamine + H2O + O2
-
Rhodococcus opacus pentanal + NH3 + H2O2
-
?
1.4.3.21 benzylamine + H2O + O2 main substrate for isoform AO1 Rhodococcus opacus benzaldehyde + NH3 + H2O2
-
?
1.4.3.21 benzylamine + H2O + O2 main substrate for isoform AO1 Rhodococcus opacus DSM 43250 benzaldehyde + NH3 + H2O2
-
?
1.4.3.21 butylamine + H2O + O2
-
Rhodococcus opacus butanal + NH3 + H2O2
-
?
1.4.3.21 dopamine + H2O + O2
-
Rhodococcus opacus (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 dopamine + H2O + O2
-
Rhodococcus opacus DSM 43250 (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 ethanolamine + H2O + O2
-
Rhodococcus opacus glycolaldehyde + NH3 + H2O2
-
?
1.4.3.21 ethanolamine + H2O + O2
-
Rhodococcus opacus DSM 43250 glycolaldehyde + NH3 + H2O2
-
?
1.4.3.21 ethylamine + H2O + O2 isoforms AO1 and AO2 show 100% activity with ethylamine Rhodococcus opacus acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 histamine + H2O + O2
-
Rhodococcus opacus 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2
-
?
1.4.3.21 methylamine + H2O + O2
-
Rhodococcus opacus formaldehyde + NH3 + H2O2
-
?
1.4.3.21 methylbenzylamine + H2O + O2
-
Rhodococcus opacus methylbenzaldehyde + NH3 + H2O2
-
?
1.4.3.21 additional information isoform AO1 isolated from butylamine-induced cells with a distinct specificity towards benzylamine (relative to phenylethylamine), acts on a broad range of aliphatic monoamines (C1-C5). Isoform AO2 isolated from phenylethylamine-treated cells displays activity towards phenylethylamine as well as tyramine and heterocyclic amines but negligible conversion of benzylamine and short chain aliphatic amines. Neither AO1 nor AO2 exhibit any measurable activity when using diamines (putrescine or cadaverine) as substrate Rhodococcus opacus ?
-
?
1.4.3.21 additional information isoform AO1 isolated from butylamine-induced cells with a distinct specificity towards benzylamine (relative to phenylethylamine), acts on a broad range of aliphatic monoamines (C1-C5). Isoform AO2 isolated from phenylethylamine-treated cells displays activity towards phenylethylamine as well as tyramine and heterocyclic amines but negligible conversion of benzylamine and short chain aliphatic amines. Neither AO1 nor AO2 exhibit any measurable activity when using diamines (putrescine or cadaverine) as substrate Rhodococcus opacus DSM 43250 ?
-
?
1.4.3.21 octopamine + H2O + O2
-
Rhodococcus opacus hydroxy(hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 phenylethylamine + H2O + O2 main substrate for isoform AO2 Rhodococcus opacus phenylacetaldehyde + NH3 + H2O2
-
?
1.4.3.21 propylamine + H2O + O2
-
Rhodococcus opacus propanal + NH3 + H2O2
-
?
1.4.3.21 sec-butylamine + H2O + O2
-
Rhodococcus opacus butan-2-one + NH3 + H2O2
-
?
1.4.3.21 tyramine + H2O + O2
-
Rhodococcus opacus (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 tyramine + H2O + O2
-
Rhodococcus opacus DSM 43250 (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.21 vanillylamine + H2O + O2
-
Rhodococcus opacus vanillic aldehyde + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.21 homodimer 2 * 70000, isoform AO1 or AO2, SDS-PAGE Rhodococcus opacus
1.4.3.21 homodimer 2 * 71695, isoform AO1, calculated from amino acid sequence Rhodococcus opacus
1.4.3.21 homodimer 2 * 72051, isoform AO2, calculated from amino acid sequence Rhodococcus opacus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 AO1 isoform Rhodococcus opacus
1.4.3.21 AO2 isoform Rhodococcus opacus
1.4.3.21 copper-containing amine oxidase
-
Rhodococcus opacus
1.4.3.21 CuAO
-
Rhodococcus opacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 0.4
-
methylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 1
-
vanillylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 1.9
-
dopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 3.3
-
ethanolamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 3.5
-
octopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 4.3
-
Butylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 5.9
-
amylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 7.2
-
ethylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 12.1
-
benzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 12.7
-
amylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 13.2
-
histamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 15.3
-
Butylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 16.1
-
tyramine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 17.3
-
4-fluorobenzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 18.9
-
Propylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 20.9
-
Phenylethylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
Rhodococcus opacus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.21 0.4
-
tyramine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.6
-
Phenylethylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.6
-
octopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 0.8
-
4-fluorobenzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 1.4
-
dopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 2.9
-
vanillylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 3.1
-
amylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 4.1
-
benzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 9.9
-
Butylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.21 Rhodococcus opacus isoform AO1, calculated from amino acid sequence
-
4.6
1.4.3.21 Rhodococcus opacus isoform AO2, calculated from amino acid sequence
-
4.9

Expression

EC Number Organism Comment Expression
1.4.3.21 Rhodococcus opacus enzyme expression is induced by limiting the nitrogen source to butylamine (isoform AO1) and phenylethylamine (isoform AO2) up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 2
-
methylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 2
-
ethanolamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 8
-
ethylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 13
-
Butylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 19
-
dopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 39
-
Propylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 47
-
histamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 48
-
Butylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 50
-
vanillylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 54
-
amylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 79
-
amylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 116
-
octopamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 133
-
4-fluorobenzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 303
-
benzylamine isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 322
-
tyramine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus
1.4.3.21 1045
-
Phenylethylamine isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C Rhodococcus opacus