EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.21 | 4-fluorobenzylamine | - |
Rhodococcus opacus | |
1.4.3.21 | aminoguanidine | isoform AO1 shows 18% residual activity at 0.1 mM, isoform AO2 shows 17% residual activity at 0.1 mM | Rhodococcus opacus | |
1.4.3.21 | amylamine | - |
Rhodococcus opacus | |
1.4.3.21 | benzylamine | - |
Rhodococcus opacus | |
1.4.3.21 | Butylamine | - |
Rhodococcus opacus | |
1.4.3.21 | Cuprizone | isoform AO1 shows 2% residual activity at 0.1 mM, isoform AO2 shows 1% residual activity at 0.1 mM | Rhodococcus opacus | |
1.4.3.21 | dopamine | - |
Rhodococcus opacus | |
1.4.3.21 | isoniazid | isoform AO1 shows 14% residual activity at 0.1 mM, isoform AO2 shows 16% residual activity at 0.1 mM | Rhodococcus opacus | |
1.4.3.21 | additional information | not inhibited by pargyline, clorgyline, and neocupine | Rhodococcus opacus | |
1.4.3.21 | octopamine | - |
Rhodococcus opacus | |
1.4.3.21 | Phenylethylamine | - |
Rhodococcus opacus | |
1.4.3.21 | Propylamine | - |
Rhodococcus opacus | |
1.4.3.21 | Semicarbazide | isoform AO1 shows 1% residual activity at 0.1 mM, isoform AO2 shows 2% residual activity at 0.1 mM | Rhodococcus opacus | |
1.4.3.21 | tyramine | - |
Rhodococcus opacus | |
1.4.3.21 | vanillylamine | - |
Rhodococcus opacus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.21 | 0.02 | - |
vanillylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.02 | - |
Phenylethylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.03 | - |
octopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.04 | - |
benzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.05 | - |
tyramine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.1 | - |
dopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.11 | - |
amylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.13 | - |
4-fluorobenzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.16 | - |
amylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.18 | - |
methylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.28 | - |
histamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.32 | - |
Butylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.32 | - |
Butylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.49 | - |
Propylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.86 | - |
ethylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 2.05 | - |
ethanolamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.21 | Cu2+ | contains copper | Rhodococcus opacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.21 | 70000 | - |
2 * 70000, isoform AO1 or AO2, SDS-PAGE | Rhodococcus opacus |
1.4.3.21 | 71695 | - |
2 * 71695, isoform AO1, calculated from amino acid sequence | Rhodococcus opacus |
1.4.3.21 | 72051 | - |
2 * 72051, isoform AO2, calculated from amino acid sequence | Rhodococcus opacus |
1.4.3.21 | 140000 | - |
gel filtration | Rhodococcus opacus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.21 | Rhodococcus opacus | - |
- |
- |
1.4.3.21 | Rhodococcus opacus DSM 43250 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.21 | ammonium sulfate precipitation, Hi-Prep 16 10 Q column chromatography, Mono Q column chromatography, and Superose 12 gel filtration | Rhodococcus opacus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.3.21 | 0.039 | - |
isoform AO1, crude extract, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
1.4.3.21 | 0.144 | - |
isoform AO2, crude extract, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
1.4.3.21 | 6.302 | - |
isoform AO1, after 161.5fold purification, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
1.4.3.21 | 13.14 | - |
isoform AO2, after 91.3fold purification, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.21 | 1-methyl-3-phenylpropylamine + H2O + O2 | - |
Rhodococcus opacus | 4-phenylbutan-2-one + NH3 + H2O2 | - |
? | |
1.4.3.21 | 4-fluorobenzylamine + H2O + O2 | - |
Rhodococcus opacus | 4-fluorobenzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | amphetamine + H2O + O2 | - |
Rhodococcus opacus | 1-phenylpropan-2-one + NH3 + H2O2 | - |
? | |
1.4.3.21 | amylamine + H2O + O2 | - |
Rhodococcus opacus | pentanal + NH3 + H2O2 | - |
? | |
1.4.3.21 | benzylamine + H2O + O2 | main substrate for isoform AO1 | Rhodococcus opacus | benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | benzylamine + H2O + O2 | main substrate for isoform AO1 | Rhodococcus opacus DSM 43250 | benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | butylamine + H2O + O2 | - |
Rhodococcus opacus | butanal + NH3 + H2O2 | - |
? | |
1.4.3.21 | dopamine + H2O + O2 | - |
Rhodococcus opacus | (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | dopamine + H2O + O2 | - |
Rhodococcus opacus DSM 43250 | (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | ethanolamine + H2O + O2 | - |
Rhodococcus opacus | glycolaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | ethanolamine + H2O + O2 | - |
Rhodococcus opacus DSM 43250 | glycolaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | ethylamine + H2O + O2 | isoforms AO1 and AO2 show 100% activity with ethylamine | Rhodococcus opacus | acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | histamine + H2O + O2 | - |
Rhodococcus opacus | 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | methylamine + H2O + O2 | - |
Rhodococcus opacus | formaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | methylbenzylamine + H2O + O2 | - |
Rhodococcus opacus | methylbenzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | additional information | isoform AO1 isolated from butylamine-induced cells with a distinct specificity towards benzylamine (relative to phenylethylamine), acts on a broad range of aliphatic monoamines (C1-C5). Isoform AO2 isolated from phenylethylamine-treated cells displays activity towards phenylethylamine as well as tyramine and heterocyclic amines but negligible conversion of benzylamine and short chain aliphatic amines. Neither AO1 nor AO2 exhibit any measurable activity when using diamines (putrescine or cadaverine) as substrate | Rhodococcus opacus | ? | - |
? | |
1.4.3.21 | additional information | isoform AO1 isolated from butylamine-induced cells with a distinct specificity towards benzylamine (relative to phenylethylamine), acts on a broad range of aliphatic monoamines (C1-C5). Isoform AO2 isolated from phenylethylamine-treated cells displays activity towards phenylethylamine as well as tyramine and heterocyclic amines but negligible conversion of benzylamine and short chain aliphatic amines. Neither AO1 nor AO2 exhibit any measurable activity when using diamines (putrescine or cadaverine) as substrate | Rhodococcus opacus DSM 43250 | ? | - |
? | |
1.4.3.21 | octopamine + H2O + O2 | - |
Rhodococcus opacus | hydroxy(hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | phenylethylamine + H2O + O2 | main substrate for isoform AO2 | Rhodococcus opacus | phenylacetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | propylamine + H2O + O2 | - |
Rhodococcus opacus | propanal + NH3 + H2O2 | - |
? | |
1.4.3.21 | sec-butylamine + H2O + O2 | - |
Rhodococcus opacus | butan-2-one + NH3 + H2O2 | - |
? | |
1.4.3.21 | tyramine + H2O + O2 | - |
Rhodococcus opacus | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | tyramine + H2O + O2 | - |
Rhodococcus opacus DSM 43250 | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.21 | vanillylamine + H2O + O2 | - |
Rhodococcus opacus | vanillic aldehyde + NH3 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.21 | homodimer | 2 * 70000, isoform AO1 or AO2, SDS-PAGE | Rhodococcus opacus |
1.4.3.21 | homodimer | 2 * 71695, isoform AO1, calculated from amino acid sequence | Rhodococcus opacus |
1.4.3.21 | homodimer | 2 * 72051, isoform AO2, calculated from amino acid sequence | Rhodococcus opacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.21 | AO1 | isoform | Rhodococcus opacus |
1.4.3.21 | AO2 | isoform | Rhodococcus opacus |
1.4.3.21 | copper-containing amine oxidase | - |
Rhodococcus opacus |
1.4.3.21 | CuAO | - |
Rhodococcus opacus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.21 | 0.4 | - |
methylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 1 | - |
vanillylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 1.9 | - |
dopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 3.3 | - |
ethanolamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 3.5 | - |
octopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 4.3 | - |
Butylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 5.9 | - |
amylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 7.2 | - |
ethylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 12.1 | - |
benzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 12.7 | - |
amylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 13.2 | - |
histamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 15.3 | - |
Butylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 16.1 | - |
tyramine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 17.3 | - |
4-fluorobenzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 18.9 | - |
Propylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 20.9 | - |
Phenylethylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.21 | 2,4,5-trihydroxyphenylalanine quinone | - |
Rhodococcus opacus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.21 | 0.4 | - |
tyramine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.6 | - |
Phenylethylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.6 | - |
octopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 0.8 | - |
4-fluorobenzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 1.4 | - |
dopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 2.9 | - |
vanillylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 3.1 | - |
amylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 4.1 | - |
benzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 9.9 | - |
Butylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.4.3.21 | Rhodococcus opacus | isoform AO1, calculated from amino acid sequence | - |
4.6 |
1.4.3.21 | Rhodococcus opacus | isoform AO2, calculated from amino acid sequence | - |
4.9 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.4.3.21 | Rhodococcus opacus | enzyme expression is induced by limiting the nitrogen source to butylamine (isoform AO1) and phenylethylamine (isoform AO2) | up |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.21 | 2 | - |
methylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 2 | - |
ethanolamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 8 | - |
ethylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 13 | - |
Butylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 19 | - |
dopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 39 | - |
Propylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 47 | - |
histamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 48 | - |
Butylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 50 | - |
vanillylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 54 | - |
amylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 79 | - |
amylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 116 | - |
octopamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 133 | - |
4-fluorobenzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 303 | - |
benzylamine | isoform AO1, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 322 | - |
tyramine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus | |
1.4.3.21 | 1045 | - |
Phenylethylamine | isoform AO2, in 200 mM potassium phosphate buffer pH 7.6, at 30°C | Rhodococcus opacus |