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Literature summary extracted from

  • Giovannini, P.; Mantovani, M.; Grandini, A.; Medici, A.; Pedrini, P.
    New acetoin reductases from Bacillus stearothermophilus: meso- and 2R,3R-butanediol as fermentation products (2011), J. Mol. Catal. B, 69, 15-20.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.304 diacetyl + NADH + H+ Geobacillus stearothermophilus
-
 (S)-acetoin + NAD+
-
 ?
1.1.1.304 diacetyl + NADH + H+ Geobacillus stearothermophilus ATCC 2027
-
 (S)-acetoin + NAD+
-
 ?
1.1.1.304 additional information Geobacillus stearothermophilus the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 ?
-
 ?
1.1.1.304 additional information Geobacillus stearothermophilus ATCC 2027 the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.304 Geobacillus stearothermophilus
-
-
-
1.1.1.304 Geobacillus stearothermophilus ATCC 2027
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.304 native enzyme by adsorption on diethylaminoethyl–Sepharose and hydrophobic interaction chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.304 diacetyl + NADH + H+
-
 Geobacillus stearothermophilus (S)-acetoin + NAD+
-
 ?
1.1.1.304 diacetyl + NADH + H+
-
 Geobacillus stearothermophilus ATCC 2027 (S)-acetoin + NAD+
-
 ?
1.1.1.304 additional information the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 Geobacillus stearothermophilus ?
-
 ?
1.1.1.304 additional information the enzyme shows an S-enantioselectivity in the reversible reduction of acetoin so it might be responsible of the meso-butenediol formation from R-acetoin. It acts on racemic acetoin and (S)-acetoin to form (2S,3S)-butane-2,3-diol, EC 1.1.1.76, but also on the (2R,3R)-butane-2,3-diol isomer in the reverse reaction, EC 1.1.1.4 Geobacillus stearothermophilus ATCC 2027 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.304 BSDR
-
 Geobacillus stearothermophilus
1.1.1.304 S-stereospecific diacetyl reductase
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.304 6.5
-
 assay at Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.304 NADH dependent on Geobacillus stearothermophilus

General Information

EC Number General Information Comment Organism
1.1.1.304 metabolism the enzyme is involved in the butanediol cycle, overview Geobacillus stearothermophilus