EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.39 | gene bphC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
1.13.11.39 | gene bphC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
1.13.11.39 | gene bphC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.39 | Ca2+ | - |
Achromobacter sp. | |
1.13.11.39 | Co2+ | - |
Achromobacter sp. | |
1.13.11.39 | Cu2+ | - |
Achromobacter sp. | |
1.13.11.39 | Fe2+ | - |
Achromobacter sp. | |
1.13.11.39 | Fe3+ | slight inhibition | Achromobacter sp. | |
1.13.11.39 | Mg2+ | - |
Achromobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.39 | 0.0021 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.00213 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0049 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0062 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.01672 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0273 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0391 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0616 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.0976 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.1306 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.2824 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 0.3605 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.39 | Fe2+ | activates 270% | Achromobacter sp. | |
1.13.11.39 | additional information | no effect by Fe3+, Mg2+ and Ca2+ | Achromobacter sp. | |
1.13.11.39 | additional information | no effect by Fe3+, Mg2+ and Ca2+, poor activating effect by Fe2+ | Achromobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.39 | biphenyl-2,3-diol + O2 | Achromobacter sp. | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | Achromobacter sp. BP3 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.39 | Achromobacter sp. | - |
isolated from petroleum-contaminated soil, gene bphC3 | - |
1.13.11.39 | Achromobacter sp. | B3VTK1 | BphC1; isolated from petroleum-contaminated soil, gene bphC1 | - |
1.13.11.39 | Achromobacter sp. | B3VTL5 | BphC2; isolated from petroleum-contaminated soil, gene bphC2 | - |
1.13.11.39 | Achromobacter sp. BP3 | - |
isolated from petroleum-contaminated soil, gene bphC3 | - |
1.13.11.39 | Achromobacter sp. BP3 | B3VTK1 | BphC1; isolated from petroleum-contaminated soil, gene bphC1 | - |
1.13.11.39 | Achromobacter sp. BP3 | B3VTL5 | BphC2; isolated from petroleum-contaminated soil, gene bphC2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.39 | 3-methylcatechol + O2 | - |
Achromobacter sp. | ? | - |
? | |
1.13.11.39 | 3-methylcatechol + O2 | - |
Achromobacter sp. BP3 | ? | - |
? | |
1.13.11.39 | 4-methylcatechol + O2 | - |
Achromobacter sp. | ? | - |
? | |
1.13.11.39 | 4-methylcatechol + O2 | - |
Achromobacter sp. BP3 | ? | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | - |
Achromobacter sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | highly preferred substrate | Achromobacter sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | - |
Achromobacter sp. BP3 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | biphenyl-2,3-diol + O2 | highly preferred substrate | Achromobacter sp. BP3 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
1.13.11.39 | catechol + O2 | - |
Achromobacter sp. | cis,cis-muconate | - |
? | |
1.13.11.39 | catechol + O2 | - |
Achromobacter sp. BP3 | cis,cis-muconate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.39 | 2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Achromobacter sp. |
1.13.11.39 | BphC | - |
Achromobacter sp. |
1.13.11.39 | BphC1 | - |
Achromobacter sp. |
1.13.11.39 | BphC2 | - |
Achromobacter sp. |
1.13.11.39 | BphC3 | - |
Achromobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 30 | - |
- |
Achromobacter sp. |
1.13.11.39 | 40 | - |
- |
Achromobacter sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 10 | 60 | activity range, profile overview | Achromobacter sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.39 | 1.19 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 3.25 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 7.36 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 8.78 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 9.87 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 10.15 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 12.02 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 21.63 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 43.2 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 52.56 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 53.83 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 86.32 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 8 | - |
- |
Achromobacter sp. |
1.13.11.39 | 8 | 9 | - |
Achromobacter sp. |
1.13.11.39 | 9 | - |
- |
Achromobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.39 | 5 | 10 | activity range, profile overview | Achromobacter sp. |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.13.11.39 | Achromobacter sp. | gene bphC2 is constitutively expressed in strain BP3 | up |
1.13.11.39 | Achromobacter sp. | gene bphC3 is induced by both biphenyl and toluene | up |
1.13.11.39 | Achromobacter sp. | the bphC1 gene is only induced by biphenyl | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.39 | metabolism | BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview | Achromobacter sp. |
1.13.11.39 | metabolism | BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview | Achromobacter sp. |
1.13.11.39 | metabolism | BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview | Achromobacter sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.39 | 4.21 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 9 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 92 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 101.1 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 164.8 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 224.6 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 440.2 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 1582 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 8477 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 10300 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 10990 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
1.13.11.39 | 40530 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. |