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Literature summary extracted from

  • Deutsch, C.; El Yacoubi, B.; de Crecy-Lagard, V.; Iwata-Reuyl, D.
    Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside (2012), J. Biol. Chem., 287, 13666-13673.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.87 yrdC, yjeE and yeaZ genes from Escherichia coli expressed as N-terminal His6-fusion proteins, YgjD N-terminal as His6-SUMO fusion protein in Escherichia coli Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.234 Escherichia coli P05852
-
-
2.7.7.87 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.87 L-threonine + ATP + bicarbonate beside YrdC, proteins YgjD, YjeE and YeaZ are necessary and sufficient for t6A biosynthesis in vitro Escherichia coli L-threonylcarbamoyladenylate + diphosphate + H2O L-threonylcarbamoyladenylate i.e. t6A ?
2.7.7.87 additional information t6A is a universally conserved tRNA modification found at position 37 in the anticodon loop of a subset of tRNA, structural studies predict an important role for t6A in translational fidelity Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.234 tRNA N6-adenosine threonylcarbamoyltransferase
-
 Escherichia coli
2.3.1.234 TsaD
-
 Escherichia coli
2.3.1.234 ygjD
-
 Escherichia coli
2.7.7.87 YrdC
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.3.1.234 physiological function proteins YgjD, YrdC, YjeE, and YeaZ are both necessary and sufficient for threonylcarbamoyl adenosine biosynthesis in vitro. tRNA N6-adenosine threonylcarbamoyltransferase YgjD binds to YjeE, but not to YeaZ Escherichia coli
2.7.7.87 physiological function t6A is a universally conserved tRNA modification found at position 37 in the anticodon loop of a subset of tRNA, structural studies predict an important role for t6A in translational fidelity Escherichia coli