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Literature summary extracted from

  • Zhou, X.; Green, A.; Singh, S.
    Caenorhabditis elegans ?12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the DELTA12 and DELTA15 positions (2011), J. Biol. Chem., 286, 43644-43650.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.22 CeFAT-2 is expressed under the control of an inducible Gal1 promoter in Saccharomyces cerevisiae Caenorhabditis elegans
1.14.19.39 gene fat-2, sequence comparison and phylogenetic tree, expression in Saccharomyces cerevisiae strain S288C Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.6 Caenorhabditis elegans G5EGA5
-
-
1.14.19.22 Caenorhabditis elegans
-
-
-
1.14.19.39 Caenorhabditis elegans G5EGA5 gene fat-2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.6 heptadecyloleic acid + reduced acceptor + O2
-
 Caenorhabditis elegans C17:2DELTA9,12 + acceptor + H2O
-
 ?
1.14.19.6 additional information the enzyme can also catalyze DELTA15 desaturation Caenorhabditis elegans ?
-
 ?
1.14.19.6 myristoleic acid + reduced acceptor + O2
-
 Caenorhabditis elegans C14:2DELTA9,12 + acceptor + H2O
-
 ?
1.14.19.6 oleic acid + reduced acceptor + O2
-
 Caenorhabditis elegans linoleic acid + acceptor + H2O
-
 ?
1.14.19.6 palmitoleic acid + reduced acceptor + O2
-
 Caenorhabditis elegans (9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
 ?
1.14.19.6 pentadecyloleic acid + reduced acceptor + O2
-
 Caenorhabditis elegans C15:2DELTA9,12 + acceptor + H2O + acceptor
-
 ?
1.14.19.22 additional information CeFAT-2 displays characteristics of a bifunctional DELTA12/DELTA15-desaturase and has a great deal of elasticity with respect to fatty acid chain length in being able to desaturate fatty acids ranging from C14 to C18 at the DELTA12 and DELTA15 positions Caenorhabditis elegans ?
-
 ?
1.14.19.39 linoleate + AH2 + O2
-
 Caenorhabditis elegans crepenynate + A + 2 H2O
-
 ?
1.14.19.39 additional information the enzyme is able to desaturate C16 and C18 fatty acids. When recombinantly expressed in yeast cells, the enzyme can also catalyze DELTA15 desaturation. The recombinant enzyme can also produce C14:2DELTA9,12, C15:2DELTA9,12, C17:2DELTA9,12, and C18:4DELTA6,9,12,15 when C14:1DELTA9, C15:1DELTA9, C17:1DELTA9, and C18:3DELTA6,9,12 substrates are available in yeast cells, product mass spectrometry analysis Caenorhabditis elegans ?
-
 ?
1.14.19.39 oleate + AH2 + O2 recombinant enzyme Caenorhabditis elegans linoleic acid + linolenic acid + A + 2 H2O
-
 ?
1.14.19.39 palmitoleic acid + O2 recombinant enzyme Caenorhabditis elegans hexadecadienoic acid + hexadecatrienoic acid + A + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.6 DELTA12-desaturase
-
 Caenorhabditis elegans
1.14.19.6 FAT-2 a bifunctional DELTA12/DELTA15-desaturase Caenorhabditis elegans
1.14.19.22 CeFAT-2
-
 Caenorhabditis elegans
1.14.19.39 DELTA12-desaturase
-
 Caenorhabditis elegans
1.14.19.39 FAT-2
-
 Caenorhabditis elegans
1.14.19.39 fatty acid DELTA12-desaturase
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
1.14.19.22 physiological function when expressed in yeast cells, CeFAT-2 displays characteristics of a bifunctional DELTA12/DELTA15-desaturase and has a great deal of elasticity with respect to fatty acid chain length in being able to desaturate fatty acids ranging from C14 to C18 Caenorhabditis elegans