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Literature summary extracted from

  • Li, Q.A.; Mavrodi, D.V.; Thomashow, L.S.; Roessle, M.; Blankenfeldt, W.
    Ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (ADIC) synthase PhzE (2011), J. Biol. Chem., 286, 18213-18221.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.86 His-tagged protein expressed in Escherichia coli BL21(DE3) Burkholderia lata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.86 hanging-drop vapor diffusion method, PEG 3350 Burkholderia lata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.86 Mn2+ reduced activity Burkholderia lata
2.6.1.86 Ni2+ reduced activity Burkholderia lata
2.6.1.86 Zn2+ reduced activity Burkholderia lata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.86 0.0024
-
 chorismate pH 7.0, 25°C Burkholderia lata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.86 Mg2+
-
 Burkholderia lata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.86 70000
-
 2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals Burkholderia lata

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.86 Burkholderia lata
-
-
-
2.6.1.86 Burkholderia lata 383
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.86 immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration Burkholderia lata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.86 L-glutamine + chorismate
-
 Burkholderia lata (S)-2-amino-2-deoxyisochorismate + L-glutamate
-
 ?
2.6.1.86 L-glutamine + chorismate
-
 Burkholderia lata 383 (S)-2-amino-2-deoxyisochorismate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.86 homodimer 2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals Burkholderia lata

Synonyms

EC Number Synonyms Comment Organism
2.6.1.86 2-amino-2desoxyisochorismate synthase
-
 Burkholderia lata
2.6.1.86 PhzE
-
 Burkholderia lata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.86 0.01
-
 chorismate pH 7.0, 25°C Burkholderia lata