EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.96 | spermidine | 0.8 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr | Pyrococcus abyssi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.96 | expression in Escherichia coli | Archaeoglobus fulgidus |
3.1.1.96 | expression in Escherichia coli, the archaeal protein forms inclusion bodies | Saccharolobus solfataricus |
3.1.1.96 | overexpression in Escherichia coli | Pyrococcus abyssi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.96 | E137A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 the enzyme displays an activity 10fold lower than that of the wild type. The mutant enzyme contains onyl one Zn2+ | Archaeoglobus fulgidus |
3.1.1.96 | E154A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ | Archaeoglobus fulgidus |
3.1.1.96 | H140A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 activity of the H140A variant is so small that it can not be detected, even with 70 nM enzyme in the assay | Archaeoglobus fulgidus |
3.1.1.96 | H141A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 variant exhibits the same activity as that of the wild-type protein | Archaeoglobus fulgidus |
3.1.1.96 | H197A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity | Archaeoglobus fulgidus |
3.1.1.96 | H80A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ | Archaeoglobus fulgidus |
3.1.1.96 | H92A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity | Archaeoglobus fulgidus |
3.1.1.96 | S78A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity | Archaeoglobus fulgidus |
3.1.1.96 | W162A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity | Archaeoglobus fulgidus |
3.1.1.96 | Y198A | in presence of 0.01 mM Zn2+ and 4 mM MgCl2 variant exhibits the same activity as that of the wild-type protein | Archaeoglobus fulgidus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.96 | EDTA | - |
Archaeoglobus fulgidus | |
3.1.1.96 | EDTA | - |
Pyrococcus abyssi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.96 | KCl | 200 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr | Pyrococcus abyssi | |
3.1.1.96 | Mg2+ | markedly improves initial rate of D-Tyr-tRNATyr hydrolysis. Optimal concentration: 16 mM | Pyrococcus abyssi | |
3.1.1.96 | Zn2+ | contains strongly bound Zn2+ | Pyrococcus abyssi | |
3.1.1.96 | Zn2+ | wild-type the enzyme contains two firmly bound Zn2+ | Archaeoglobus fulgidus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.96 | 26959 | - |
x * 26959, calculated from sequence | Saccharolobus solfataricus |
3.1.1.96 | 30000 | - |
x * 30000, SDS-PAGE | Saccharolobus solfataricus |
3.1.1.96 | 30781 | - |
1 * 30781, calculated from sequence | Pyrococcus abyssi |
3.1.1.96 | 30907 | - |
1 * 30907, mass spectrometry, SDS-PAGE | Pyrococcus abyssi |
3.1.1.96 | 35000 | - |
gel filtration | Pyrococcus abyssi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | Saccharolobus solfataricus | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | Pyrococcus abyssi | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | Archaeoglobus fulgidus | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | Saccharolobus solfataricus P2 | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | D-tyrosine + tRNATyr | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.96 | Archaeoglobus fulgidus | O29630 | - |
- |
3.1.1.96 | Pyrococcus abyssi | Q9V2R8 | - |
- |
3.1.1.96 | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | Q9V2R8 | - |
- |
3.1.1.96 | Saccharolobus solfataricus | Q97WI2 | - |
- |
3.1.1.96 | Saccharolobus solfataricus P2 | Q97WI2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.96 | - |
Saccharolobus solfataricus |
3.1.1.96 | - |
Pyrococcus abyssi |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.96 | -20°C, 20 mM Tris-HCl buffer, pH 7.8, containing 60% glycerol and 0.05 mM zinc acetate | Saccharolobus solfataricus |
3.1.1.96 | -20°C, 20 mM Tris-HCl buffer, pH 7.8, containing 60% glycerol and 0.05 mM zinc acetate | Pyrococcus abyssi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | - |
Saccharolobus solfataricus | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | - |
Archaeoglobus fulgidus | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | Saccharolobus solfataricus | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | Pyrococcus abyssi | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | Archaeoglobus fulgidus | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr | Pyrococcus abyssi | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | - |
Saccharolobus solfataricus P2 | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | Saccharolobus solfataricus P2 | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the enzyme can recycle misaminoacylated D-Tyr-tRNATyr | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | D-tyrosine + tRNATyr | - |
? | |
3.1.1.96 | D-tyrosyl-tRNATyr + H2O | the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | D-tyrosine + tRNATyr | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.96 | ? | x * 30000, SDS-PAGE | Saccharolobus solfataricus |
3.1.1.96 | ? | x * 26959, calculated from sequence | Saccharolobus solfataricus |
3.1.1.96 | monomer | 1 * 30781, calculated from sequence | Pyrococcus abyssi |
3.1.1.96 | monomer | 1 * 30907, mass spectrometry, SDS-PAGE | Pyrococcus abyssi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.96 | D-Tyr-tRNATyr deacylase | - |
Saccharolobus solfataricus |
3.1.1.96 | D-Tyr-tRNATyr deacylase | - |
Pyrococcus abyssi |
3.1.1.96 | D-Tyr-tRNATyr deacylase | - |
Archaeoglobus fulgidus |
3.1.1.96 | dtd2 | - |
Archaeoglobus fulgidus |
3.1.1.96 | dtd2 | - |
Saccharolobus solfataricus |
3.1.1.96 | dtd2 | - |
Pyrococcus abyssi |
3.1.1.96 | dtdA | - |
Saccharolobus solfataricus |
3.1.1.96 | dtdA | - |
Pyrococcus abyssi |
3.1.1.96 | SS02234 | locus name | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.96 | 37 | - |
assay at | Saccharolobus solfataricus |
3.1.1.96 | 37 | - |
assay at | Pyrococcus abyssi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.96 | 7.8 | - |
assay at | Saccharolobus solfataricus |
3.1.1.96 | 7.8 | - |
assay at | Pyrococcus abyssi |