EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.7.15 | reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment | Rhodobacter capsulatus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.15 | [4Fe-4S]-center | - |
Rhodobacter capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.15 | chlorophyllide a + AH2 + ATP + H2O | Rhodobacter capsulatus | - |
3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.7.15 | Rhodobacter capsulatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.7.15 | - |
Rhodobacter capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.15 | chlorophyllide a + AH2 + ATP + H2O | - |
Rhodobacter capsulatus | 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? | |
1.3.7.15 | chlorophyllide a + AH2 + ATP + H2O | the enzyme catalyses trans-reduction of the B-ring of chlorophyllide a. The product has the (7R,8R)-configuration | Rhodobacter capsulatus | 3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.7.15 | BchX | - |
Rhodobacter capsulatus |
1.3.7.15 | BchY | - |
Rhodobacter capsulatus |
1.3.7.15 | BchZ | - |
Rhodobacter capsulatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.7.15 | 34 | - |
assay at | Rhodobacter capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.7.15 | 8 | - |
assay at | Rhodobacter capsulatus |