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Literature summary extracted from

  • Chen, Y.H.; Kocherginskaya, S.A.; Lin, Y.; Sriratana, B.; Lagunas, A.M.; Robbins, J.B.; Mackie, R.I.; Cann, I.K.
    Biochemical and mutational analyses of a unique clamp loader complex in the archaeon Methanosarcina acetivorans (2005), J. Biol. Chem., 280, 41852-41863.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.4.B8 dsDNA the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 12fold by dsDNA Methanosarcina acetivorans
3.6.4.B8 proliferating cell nuclear antigen the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 3fold by Methanosarcina acetivorans proliferating cell nuclear antigen Methanosarcina acetivorans
3.6.4.B8 singly primed single-stranded DNA the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 58fold by singly primed single-stranded DNA Methanosarcina acetivorans
3.6.4.B8 ssDNA the MacRFC complex (a protein complex of Mac-RFCS1, MacRFCS2, and MacRFCL) possesses very low intrinsic ATPase activity. This activity is stimulated about 33fold by ssDNA Methanosarcina acetivorans

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.B8 additional information site-directed mutagenesis in the Walker A and SRC motifs is performed to examine the contribution of each subunit to the function of the Methanosarcina acetivorans clamp loader. Mutations in the large subunit MacRFCL and the small subunit MacRFCS2 do not impair clamp loading activity. Any mutant clamp loader harboring a mutation in MacRFCS1 is devoid of the clamp loading property Methanosarcina acetivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.4.B8 35000
-
 1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits Methanosarcina acetivorans
3.6.4.B8 38000
-
 1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits Methanosarcina acetivorans
3.6.4.B8 67000
-
 1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits Methanosarcina acetivorans
3.6.4.B8 344500
-
 gel filtration Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B8 ATP + H2O Methanosarcina acetivorans
-
 ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O Methanosarcina acetivorans DSM 2834
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B8 Methanosarcina acetivorans Q8TSX5 and Q8TUC8 and Q8TPU4 Q8TSX5: subunit MacRFCS1, Q8TUC8: subunit MacRFCS2, Q8TPU4: subunit MacRFCL
-
3.6.4.B8 Methanosarcina acetivorans DSM 2834 Q8TSX5 and Q8TUC8 and Q8TPU4 Q8TSX5: subunit MacRFCS1, Q8TUC8: subunit MacRFCS2, Q8TPU4: subunit MacRFCL
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B8 ATP + H2O
-
 Methanosarcina acetivorans ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O the clamp loader complex reconstituted from the three subunits MacRFCS1, MacRFCS2, and MacRFCL stimulates DNA synthesis by a cognate DNA polymerase in the presence of its sliding clamp. Mac-RFCS1 is critical to the clamp loading activity of the Methanosarcina acetivorans clamp loader Methanosarcina acetivorans ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O
-
 Methanosarcina acetivorans DSM 2834 ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O the clamp loader complex reconstituted from the three subunits MacRFCS1, MacRFCS2, and MacRFCL stimulates DNA synthesis by a cognate DNA polymerase in the presence of its sliding clamp. Mac-RFCS1 is critical to the clamp loading activity of the Methanosarcina acetivorans clamp loader Methanosarcina acetivorans DSM 2834 ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.4.B8 trimer 1 * 67000 (MacRFCL) + 1 * 38000 (MacRFCS2) + 1 * 35000 (MacRFCS1), SDS-PAGE of His6-tagged subunits Methanosarcina acetivorans

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B8 MacRFC complex
-
 Methanosarcina acetivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B8 7.5
-
 assay at Methanosarcina acetivorans